Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,795,6500AC34.7% 92.2 / 13.9 49V39G (GTG→GGG) celFcryptic phospho‑beta‑glucosidase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (28/4);  new base C (0/17);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 9.21e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCT  >  REL606/1795575‑1795723
                                                                           |                                                                         
aCGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATcca                                                                           >  2:500826/1‑76 (MQ=255)
 cGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATccac                                                                          <  2:130462/76‑1 (MQ=255)
  gCCAGCGTTATCAAACATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATccacc                                                                         >  1:874987/1‑76 (MQ=255)
    cAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAg                                                                       <  2:1352953/76‑1 (MQ=255)
     aGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGc                                                                      >  2:878990/1‑76 (MQ=255)
        gTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCAt                                                                   >  1:397389/1‑76 (MQ=255)
           atcaatcaCCCGTGGGCAGAGACCGAAAATAATATCCATTTCCGCTTTCCCTCCTTCGACACCCCCCAGCCATAAt                                                                <  2:864334/76‑1 (MQ=255)
             caatcaTCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCCCCTTCGACATCCCCCAGCCATAATTc                                                              <  1:60107/76‑1 (MQ=255)
                  atcCGTTGGCGGAGATCGAAAATAATATCCAGTTTCGCTTTACCCCCTTCGACACCCCCCAGCCATAATTCGCTGa                                                         <  2:808816/76‑1 (MQ=255)
                  atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGa                                                         >  2:458475/1‑76 (MQ=255)
                  atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGa                                                         >  1:362718/1‑76 (MQ=255)
                  atcCGGTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTTCCCCCTTCGCCAACCACCAGCCATAATTCGCTGa                                                         <  2:289745/76‑1 (MQ=255)
                    ccGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGAcc                                                       >  1:1358034/1‑76 (MQ=255)
                     cGTTGGCAGAGATCNAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCg                                                      >  1:886643/1‑76 (MQ=255)
                      gTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCgg                                                     >  1:834549/1‑76 (MQ=255)
                      gTTGGCAGAGACCAAAAATAATATCCAGTTTCGCTTTCCCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCgg                                                     <  1:233345/76‑1 (MQ=255)
                       ttGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGGCCGGc                                                    >  2:1629680/1‑76 (MQ=255)
                        tGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa                                                   >  2:540644/1‑76 (MQ=255)
                        tGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa                                                   >  1:97894/1‑76 (MQ=255)
                          gCAGAGAACAAAAAAAATATCCTTTTTCGCTTTCCCGCCTTGGCCATCCACCAGCCATAATTCGCTGACCGGCAAt                                                 <  2:1348002/76‑1 (MQ=255)
                            agagATCAAAAATAATATCCAGTTTCGCTTTCCCACCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTc                                               <  1:983053/76‑1 (MQ=255)
                                aTCGAAAATAATATCCAGTTTCGCTTTACCCCCTTCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGa                                           <  1:1167836/76‑1 (MQ=255)
                                  caaaaaaaACACCCGGTCTCGCTTTCCAACCTGGCACTCCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa                                         <  2:642242/74‑1 (MQ=255)
                                   gAAAATAATATCCATTTCCGCTTTCCCCCCTCCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATaa                                        <  1:1182349/76‑1 (MQ=255)
                                   aaaaaaaATATCCAGTTTCGCTTTCCCCCCTTCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATaa                                        <  1:727979/75‑1 (MQ=255)
                                    aaaaTAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAAc                                       >  2:101557/1‑76 (MQ=255)
                                    aaaaTAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAAc                                       >  1:1130655/1‑76 (MQ=255)
                                     aaaTAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACg                                      >  2:951958/1‑76 (MQ=255)
                                      aataatATCCATTTCCGCTTTCCCCCCTTCGCCACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGc                                     <  2:618711/76‑1 (MQ=255)
                                        taataTCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCtt                                   >  1:479392/1‑76 (MQ=255)
                                        taataTCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCtt                                   >  2:926493/1‑76 (MQ=255)
                                         aataTCCAGTTTCGCTTTACCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTta                                  <  2:1619181/76‑1 (MQ=255)
                                         aataTCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTta                                  >  2:1148448/1‑76 (MQ=255)
                                         aataTCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTta                                  >  2:678178/1‑76 (MQ=255)
                                          atgtCCCGTTTCCCTTCACCACCTGCGACACCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaa                                 <  2:1374236/73‑1 (MQ=255)
                                           tatCCATTTCCGCTTTACCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaat                                <  1:254039/76‑1 (MQ=255)
                                           tatCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaat                                >  2:1151911/1‑76 (MQ=255)
                                            atCCAGTTTCGCTTTACCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaata                               <  1:619902/76‑1 (MQ=255)
                                             cccAGTTTCCCTTTCCCCCCTCCGCCACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaataa                              <  1:1143452/75‑1 (MQ=255)
                                               cattttCGCTTCACCCCCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAAt                            <  2:659916/73‑1 (MQ=255)
                                                   ttCGCTTTACCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCtt                        <  2:1090171/76‑1 (MQ=255)
                                                    tCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTc                       >  2:963495/1‑76 (MQ=255)
                                                     cGCTTTCCCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTcc                      <  2:1358034/76‑1 (MQ=255)
                                                       cTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAg                    >  2:950360/1‑76 (MQ=255)
                                                        tttACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGt                   >  1:924894/1‑76 (MQ=255)
                                                         ttACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTa                  >  2:543652/1‑76 (MQ=255)
                                                         ttACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTa                  >  1:558066/1‑76 (MQ=255)
                                                          tACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTaa                 >  2:433673/1‑76 (MQ=255)
                                                              cccTCCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTcc             <  1:951958/75‑1 (MQ=255)
                                                              accTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTcc             >  1:1192249/1‑76 (MQ=255)
                                                               ccTCCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCg            <  2:631550/76‑1 (MQ=255)
                                                                 ttCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCggg          <  2:96780/76‑1 (MQ=255)
                                                                 ttCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCggg          <  2:1154347/76‑1 (MQ=255)
                                                                      cacccaccaGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGtata     <  2:1567626/73‑1 (MQ=255)
                                                                       aTCCACCAGCCATAATTCGCTGACCGTCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAg    >  1:224376/1‑76 (MQ=255)
                                                                        tCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgc   >  1:431850/1‑76 (MQ=255)
                                                                        tCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgc   >  2:557774/1‑76 (MQ=255)
                                                                         ccaccaGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgct  >  2:1376664/1‑76 (MQ=255)
                                                                           |                                                                         
ACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCT  >  REL606/1795575‑1795723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑