Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	783,391	G→A	G292G (GGG→GGA)	ybhI →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	783,391	0	G	A	97.7%	159.8 / ‑3.8	43	G292G (GGG→GGA)	ybhI	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (24/18);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GCGAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGAC  >  REL606/783324‑783463                                                                    |                                                                         gcgAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTcgatga                                                                  >  2:1611296/1‑76 (MQ=255)     aaaaaTGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATcc                                                              <  2:1373628/76‑1 (MQ=255)     aaaaaTGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATcc                                                              <  1:42291/76‑1 (MQ=255)     aaaaaTGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATcc                                                              <  1:356924/76‑1 (MQ=255)        aaTGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCAcc                                                           <  1:1621560/76‑1 (MQ=255)          tgctgcTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGt                                                         <  1:333312/76‑1 (MQ=255)            ctgctgGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTgg                                                       >  2:1095723/1‑76 (MQ=255)             tgctCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGc                                                      >  2:1421577/1‑76 (MQ=255)              gctCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCa                                                     >  2:99919/1‑76 (MQ=255)                 cGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATc                                                  <  2:1496735/76‑1 (MQ=255)                      tCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCgttgt                                             >  1:11422/1‑76 (MQ=255)                       cTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCgttgtt                                            <  1:96611/76‑1 (MQ=255)                          tttgCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATg                                         >  2:1119615/3‑76 (MQ=255)                               tGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAAc                                    >  2:206756/1‑76 (MQ=255)                                 gcgcTGCTGGGCTGGATTTTCAGTAAGTCTCTGAGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCa                                  <  1:922521/76‑1 (MQ=255)                                   gctgctGGGCTGGATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCAtg                                <  1:1538093/76‑1 (MQ=255)                                       ctgggctggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCAtgctgc                            <  1:1057932/76‑1 (MQ=255)                                       ctgggctggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCAtgctgc                            >  1:1355159/1‑76 (MQ=255)                                       ctgggctggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCAggctgc                            >  2:355275/1‑76 (MQ=255)                                        tgggctggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCAtgctgct                           >  1:768349/1‑76 (MQ=255)                                        tgggctggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCAcgctgct                           <  1:697060/76‑1 (MQ=255)                                        tgggctggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACAAtgctgct                           >  2:323063/1‑76 (MQ=255)                                         gggctggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCAtgctgctg                          >  1:12885/1‑76 (MQ=255)                                            ctggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGctgctgctg                       <  2:669560/76‑1 (MQ=255)                                              ggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTggg                     >  2:330535/1‑76 (MQ=255)                                              ggATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTggg                     >  1:815347/1‑76 (MQ=255)                                               gATTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGt                    >  2:92617/1‑76 (MQ=255)                                                gttttCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTa                   >  1:855400/2‑76 (MQ=255)                                                aTTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTa                   >  2:821493/1‑76 (MQ=255)                                                aTTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTa                   >  1:35724/1‑76 (MQ=255)                                                aTTTTCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTa                   >  1:1567850/1‑76 (MQ=255)                                                 ttttCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTAt                  >  1:1271180/1‑76 (MQ=255)                                                 ttttCAGTAAGTCTCTGGGAGTTGATGAATCCCCCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTAt                  <  2:1206832/76‑1 (MQ=255)                                                   ttCAGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCg                <  1:799437/76‑1 (MQ=255)                                                      aGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTa             >  1:124954/1‑76 (MQ=255)                                                      aGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTa             >  2:1159629/1‑76 (MQ=255)                                                      aGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTa             <  1:925698/76‑1 (MQ=255)                                                      aGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTa             <  2:996533/76‑1 (MQ=255)                                                      aGTAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTCATGGCAACCATGCTGCTGCAGACTATCGCTa             >  1:428235/1‑76 (MQ=255)                                                        tAAGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTAcc           >  1:260965/1‑76 (MQ=255)                                                         aaGTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCt          <  2:73778/76‑1 (MQ=255)                                                           gTCTCTGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTgg        <  1:361385/76‑1 (MQ=255)                                                             ctctGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGa      <  2:388408/76‑1 (MQ=255)                                                             ctctGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGa      >  2:891968/1‑76 (MQ=255)                                                             ctctGGGAGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGGATCGTTACCTGGGa      >  2:891969/1‑76 (MQ=255)                                                                 gggagTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGAc  <  1:520999/76‑1 (MQ=255)                                                                    |                                                                         GCGAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGAC  >  REL606/783324‑783463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑