| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,346,924 | G→A | intergenic (+422/‑117) | yhdV → / → yhdW | predicted outer membrane protein/predicted amino‑acid transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,346,924 | 0 | G | A | 92.6% | 187.7 / 0.7 | 54 | intergenic (+422/‑117) | yhdV/yhdW | predicted outer membrane protein/predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (1/3); new base A (27/23); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGG > REL606/3346851‑3346996 | cTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGa < 2:270683/76‑1 (MQ=255) ttATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGaa < 2:374734/76‑1 (MQ=255) tataAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACaagaag < 1:596462/76‑1 (MQ=255) tataAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACaagaag < 2:1483274/76‑1 (MQ=255) ataAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGc > 1:314108/1‑76 (MQ=255) ataAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGc < 1:896557/76‑1 (MQ=255) taAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCa > 2:1193680/1‑76 (MQ=255) agagGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCAtt < 2:228162/76‑1 (MQ=255) gagGATGGCCTCAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTa < 2:1125505/76‑1 (MQ=255) gagGATGGCCCCAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTa < 2:1125518/76‑1 (MQ=255) ggATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATg < 2:1517930/76‑1 (MQ=255) gATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGa > 2:1176951/1‑76 (MQ=255) aaggCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGaa > 1:177397/3‑76 (MQ=255) aTGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGaa > 1:1553361/1‑76 (MQ=255) tGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGaaa < 1:456843/76‑1 (MQ=255) ggCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGaaaa > 1:1380059/1‑76 (MQ=255) gCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGaaaaa < 2:1503209/76‑1 (MQ=255) acagacagGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAgat < 1:625680/76‑1 (MQ=255) cagacagGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAgatg > 2:89452/1‑76 (MQ=255) cagacagGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAgatg > 1:11131/1‑76 (MQ=255) agacagGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAgatga < 1:1560515/76‑1 (MQ=255) agacagGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAgatga > 1:1618325/1‑76 (MQ=255) agacagGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAgatga > 1:552208/1‑76 (MQ=255) cagGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAgatgatga < 1:147318/76‑1 (MQ=255) cagGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAgatgatga < 2:1420448/76‑1 (MQ=255) agGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAgatgatgat < 1:480672/76‑1 (MQ=255) ggTAAAAGACGTTTGCCAAACGTGGTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATa < 1:254589/76‑1 (MQ=255) tAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGc > 2:1577241/1‑76 (MQ=255) tAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGc < 1:465881/76‑1 (MQ=255) tAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGc > 1:356698/1‑76 (MQ=255) aaaaGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGcc > 1:166656/1‑76 (MQ=255) aaaGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCca < 2:1125464/76‑1 (MQ=255) aaGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCcac > 2:201319/1‑76 (MQ=255) aGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCcaca < 1:847698/76‑1 (MQ=255) tttCCCAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGc > 2:1160691/1‑76 (MQ=255) cccAAACGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTgg > 2:237330/1‑75 (MQ=255) cAAACTTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATAATAGCCACACTGGCTgccg < 1:536263/76‑1 (MQ=255) aaCGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTgccgcc > 2:1410407/1‑76 (MQ=255) aaCGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTgccgcc < 2:1000015/76‑1 (MQ=255) cGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACGCTGGCTGGCGCAAg > 2:1546712/1‑76 (MQ=255) cGTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAg < 1:405940/76‑1 (MQ=255) gTCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGc < 2:1082110/76‑1 (MQ=255) tCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCg < 1:63154/76‑1 (MQ=255) tCCTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCg < 1:536368/76‑1 (MQ=255) cTATAACGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGGCGCCcgcggg > 2:1122030/1‑74 (MQ=255) aaCGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGtgttgc > 1:541987/1‑76 (MQ=255) aaCGATAATTTTCACCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCCGCGcgctgc > 1:1078716/1‑76 (MQ=255) cGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCtt < 1:198004/76‑1 (MQ=255) cGATAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCtt < 2:314108/76‑1 (MQ=255) aTAATTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCCGCGGGCCGCtttc > 1:248589/1‑74 (MQ=255) aTTTTCGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACCCTGGCTGCCGCCAGCGTGCTGCTTGCCGt < 2:1577752/76‑1 (MQ=255) cGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCCGCGCGCGGCCTCCCGCTGcgc > 1:478032/1‑74 (MQ=255) cGCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCaa < 2:1502973/76‑1 (MQ=255) gCCTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCCGCGGGCTGCCTACCGGCGCaaa > 2:1146470/1‑76 (MQ=255) ccTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCCCCCGCGGGCTGCTTGGCGTTGCGAAt > 1:1042915/1‑76 (MQ=255) ccTCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCCGCGCGCTGCTTGCCGTTGCcaaa > 2:1036010/1‑75 (MQ=255) tCACAAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCCCCAGCGTGCTGCTTGCCGGTGCAAATCa > 2:473067/1‑76 (MQ=255) cacaAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAg < 2:1421823/76‑1 (MQ=255) acaAGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGGGCTGCTTGCCGGTGCAAATCaag > 1:689073/1‑74 (MQ=255) | CTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGG > REL606/3346851‑3346996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |