| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 433,771 | 2 bp→AC | coding (266‑267/273 nt) | hupB → | HU, DNA‑binding transcriptional regulator, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 433,771 | 0 | T | A | 96.5% | 216.5 / ‑0.8 | 58 | V89D (GTA→GAC) | hupB | HU, DNA‑binding transcriptional regulator, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (30/26); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01 | |||||||||||
| * | REL606 | 433,772 | 0 | A | C | 94.8% | 229.5 / ‑0.5 | 58 | V89D (GTA→GAC) | hupB | HU, DNA‑binding transcriptional regulator, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/3); new base C (30/25); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 | |||||||||||
GGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCTTTTTTA > REL606/433701‑433840 || ggTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACt < 2:1067254/76‑1 (MQ=255)ggTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACt < 2:1039187/76‑1 (MQ=255) gTAAAGAGATCCCCATCGCTGCTGCTAAAGTATCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTa < 1:1377801/76‑1 (MQ=255) tAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTaa < 1:912209/76‑1 (MQ=255) aaaGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAg < 1:799561/76‑1 (MQ=255) aaaGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAg > 2:1589274/1‑76 (MQ=255) aaGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGc > 2:45612/1‑76 (MQ=255) gagaTCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGt < 2:558489/76‑1 (MQ=255) agaTCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGtt < 1:29865/76‑1 (MQ=255) gaTCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTg < 2:197047/76‑1 (MQ=255) aTCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGt > 2:1615434/1‑76 (MQ=255) aTCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGt < 1:7615/76‑1 (MQ=255) cACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTcc > 1:147233/1‑76 (MQ=255) cACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTcc > 1:405627/1‑76 (MQ=255) cATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCa < 2:1003293/76‑1 (MQ=255) aTCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAg > 2:319087/1‑76 (MQ=255) aTCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAg > 1:525578/1‑76 (MQ=255) cGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTg < 2:962578/76‑1 (MQ=255) cGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTg > 1:414226/1‑76 (MQ=255) cGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTg > 1:235703/1‑76 (MQ=255) gctAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGAtgt > 1:1348813/1‑76 (MQ=255) gctAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGAtgt > 1:408530/1‑76 (MQ=255) ctAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGAtgtg > 2:1585379/1‑76 (MQ=255) tAAAGTACCGAGCGTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGa < 2:886581/76‑1 (MQ=255) aaaGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGAc > 1:440317/1‑76 (MQ=255) aaaGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGAc > 1:187731/1‑76 (MQ=255) aaGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCGCAGTGGGGATGTGACg > 1:1535443/1‑76 (MQ=255) aGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGa > 2:319709/1‑76 (MQ=255) aGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGa > 2:561984/1‑76 (MQ=255) aGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGa > 1:952421/1‑76 (MQ=255) gTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGaa < 1:1346311/76‑1 (MQ=255) aCCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGt > 1:227686/1‑76 (MQ=255) aCCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGt > 2:618228/1‑76 (MQ=255) gAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCa < 1:1009529/76‑1 (MQ=255) cTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAgg < 2:959124/76‑1 (MQ=255) ccGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGgcg > 1:210061/1‑76 (MQ=255) ccGTGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGgcg < 1:286152/76‑1 (MQ=255) tGCAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCAt > 2:574369/1‑76 (MQ=255) cAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCt < 1:505359/76‑1 (MQ=255) cAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCt < 2:1328253/76‑1 (MQ=255) cAGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCt < 1:40045/76‑1 (MQ=255) aGGTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTa > 1:1041626/1‑76 (MQ=255) ggTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTan < 1:1071401/76‑2 (MQ=255) gTAAAGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACt < 1:976310/76‑1 (MQ=255) aaaGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGa < 2:409907/76‑1 (MQ=255) aaaGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGa < 2:1383142/76‑1 (MQ=255) aaaGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGa > 1:924036/1‑76 (MQ=255) aaGCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGAt < 1:1406836/76‑1 (MQ=255) gCACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGAtgt < 2:660396/76‑1 (MQ=255) cACTGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGAtgtg < 1:38266/76‑1 (MQ=255) tGAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCt > 1:1162410/1‑76 (MQ=255) tGAAAGACGCGGAAAGCAAAGCGTTGTCCCCAGTGGGGATGAGACGAAGTTCAAGGGCGCATCTACTGATGTGCCt < 1:830451/76‑1 (MQ=255) gAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCtt > 1:1326892/1‑76 (MQ=255) gAAAGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCtt < 2:140893/76‑1 (MQ=255) aaaGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCACGGGCGCATCTACTGATGTGCCttt > 2:172986/1‑76 (MQ=255) aaGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCtttt > 2:148375/1‑76 (MQ=255) aGACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCttttt > 1:1194173/1‑76 (MQ=255) gACGCGGACAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCtttttt > 1:322759/1‑76 (MQ=255) aCGCGGACAACTAAGCGTTGTCGCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCTTTTTTa < 1:142851/76‑1 (MQ=255) || GGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCTTTTTTA > REL606/433701‑433840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |