Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	474,106	Δ15 bp	coding (1186‑1200/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 474105	1 (0.020)	44 (1.130)	36/126	0.1	98.0%	coding (1201/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	474121 =	1 (0.030)					coding (1185/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

CCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/474020‑474105 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctcttccagcgtTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGC  >  REL606/474121‑474195                                                                                                                                                                   CCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCT                                                                                       >  2:1311259/1‑76   TACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCT                                                                                     >  2:527464/1‑76   TACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCT                                                                                     <  2:715039/76‑1      ACGACCGTAACCCAGCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCC                                                                                  <  2:1406295/76‑1      ACGACCGAAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCC                                                                                  >  1:956004/1‑76          CCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCG                                                                              <  2:685316/76‑1             TAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTT                                                                           <  1:471865/76‑1               ACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCG                                                                         <  1:704613/76‑1                   ACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCT                                                                     <  2:1106187/76‑1                    CCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTT                                                                    <  2:902639/76‑1                     CAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTC                                                                   <  2:371987/76‑1                     CAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTC                                                                   >  1:1162368/1‑76                      AGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCG                                                                  >  2:828449/1‑76                       GTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGC                                                                 <  2:898087/76‑1                        TAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCC                                                                >  2:1160501/1‑76                         AGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCA                                                               <  2:852043/76‑1                            GCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGAT                                                            >  2:1422447/1‑76                            GCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGAT                                                            >  1:190300/1‑76                              ATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATAT                                                          >  2:1156459/1‑76                              ATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATAT                                                          >  2:1299706/1‑76                              ATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATAT                                                          <  2:380097/76‑1                                GGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTT                                                        <  2:464019/76‑1                                  TTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCT                                                      <  1:1430291/76‑1                                     CAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA                                                   <  2:320218/76‑1                                        ATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTA                                                >  2:401076/1‑76                                         TATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAn                                               <  1:843710/76‑2                                          ATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGT                                              >  1:1043903/1‑76                                          ATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGCCTTCGCCAGATATTTCTCCCGTAGT                                              >  1:413448/1‑76                                           TCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG                                             >  1:1487631/1‑76                                               CTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAA                                         <  2:339059/76‑1                                                 GGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACA                                       >  1:410726/1‑76                                                 GGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGACAGATATTTCTACAGTAGTGCGAACA                                       >  2:1303672/1‑76                                                    ATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTA                                    >  1:1059894/1‑76                                                      CTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACC                                  <  1:576772/76‑1                                                             TCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGA                           <  2:551330/76‑1                                                             TCTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGA                           <  2:1229343/76‑1                                                              CTTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAG                          >  2:1180366/1‑76                                                               TTCTTCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGg                         >  2:386239/1‑75                                                               TTCTTCGATTGCCTCTTCCAGCGTTTAGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGC                         >  1:417670/1‑76                                                                   TCGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAA                     <  1:1470672/76‑1                                                                    CGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAAT                    <  2:157443/76‑1                                                                    CGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAAT                    >  2:618863/1‑76                                                                    CGATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAAT                    <  1:1432369/76‑1                                                                     GATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATC                   <  2:703074/76‑1                                                                      ATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCG                  <  2:937325/76‑1                                                                      ATTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCG                  >  2:1239516/1‑76                                                                       TTGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGA                 >  2:135003/1‑76                                                                        TGCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGAC                <  1:433835/76‑1                                                                         GCCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACA               <  1:1118994/76‑1                                                                          CCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAG              <  1:545570/76‑1                                                                             CTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAT           <  2:850676/76‑1                                                                                 CAGCGTTTCGGTCTTCGCCAGATATTTCGCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCC       <  1:130940/76‑1                                                                                    CGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCT    <  1:1341730/76‑1                                                                                    CGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCT    <  2:917457/76‑1                                                                                    CGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCT    <  1:103924/76‑1                                                                                      TTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGC  >  1:314085/1‑76                                                                                                                                                                   CCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/474020‑474105 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctcttccagcgtTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGC  >  REL606/474121‑474195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.