Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	1,305,487	IS150 (–) +3 bp	coding (751‑753/1461 nt)	cls ←	cardiolipin synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 666130	NA (NA)	45 (0.980)	36/148	0.3	97.7%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	= 1305489	1 (0.020)					coding (751/1461 nt)	cls	cardiolipin synthetase
*	?	REL606	1305487 =	1 (0.020)	46 (0.990)	37/150	0.3	97.8%	coding (753/1461 nt)	cls	cardiolipin synthetase
	?	REL606	= 2775877	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

ATCATGATCGATAATTACATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/1305558‑1305487 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA  <  REL606/2775877‑2775803                                                                                                                                                     ATCATGATCGATAATTACATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTA                                                                         <  2:1408106/76‑1    ATGATCGGTAATTACATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTG                                                                      >  1:936327/1‑76    ATGATCGATAATTACATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTG                                                                      <  1:10119/76‑1     TGATCGATAATTACATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGC                                                                     >  1:207455/1‑76     TGATCGATAATTACATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGC                                                                     <  1:479256/76‑1      GATCGATAATTACATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCA                                                                    <  1:1462810/76‑1           ATAATTACATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCAT                                                               <  2:1275050/76‑1                   ATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGG                                                       <  1:110200/76‑1                    TCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGA                                                      <  1:252150/76‑1                     CGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGAC                                                     >  1:1103556/1‑76                       CGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGA                                                   >  1:134698/1‑76                        GTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGAT                                                  >  2:1085643/1‑76                         TACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTTTACTGCACCCATTTTGTTGGACGATG                                                 >  2:1393467/1‑76                         TACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATG                                                 >  2:1245673/1‑76                         TACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATG                                                 <  1:1140579/76‑1                         TACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATG                                                 <  1:1051245/76‑1                          ACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGA                                                >  2:1169504/1‑76                              CGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATG                                            >  2:107699/1‑76                                 CAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                         <  1:669511/76‑1                                 CAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                         >  1:1303850/1‑76                                 CAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                         <  1:128469/76‑1                                  AGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT                                        >  2:601143/1‑76                                    CATGAATATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAG                                      <  2:207455/76‑1                                          TATGGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCACTA                                >  1:1410405/1‑76                                             GGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                             <  1:1484869/76‑1                                             GGTCGACCCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                             <  2:848187/76‑1                                                    CCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                      >  2:152652/1‑76                                                    CCTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                      <  2:568990/76‑1                                                     CTCGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                     <  1:1032114/76‑1                                                       CGCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCC                   >  2:1324131/1‑76                                                        GCTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCA                  <  1:1169504/76‑1                                                         CTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAA                 <  2:982873/76‑1                                                         CTACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAA                 >  2:1160938/1‑76                                                           ACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAA               >  2:1127076/1‑76                                                           ACTTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAA               >  1:672122/1‑76                                                            CTTCAAACAAGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAT              <  1:1238483/76‑1                                                             TTCAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGACATGGAAAAGCCCCTAATATGGCAAAGCCAAAATA             >  2:242711/1‑76                                                               CAAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC           <  1:394282/76‑1                                                                AAACAAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCa          <  1:1452461/76‑2                                                                    AAGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTT      <  2:1251205/76‑1                                                                      GATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA    <  2:45411/76‑1                                                                      GATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA    >  2:61707/1‑76                                                                      GATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA    >  1:574993/1‑76                                                                       ATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA   >  2:149795/1‑76                                                                        TTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA  <  1:438967/76‑1                                                                        TTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA  <  2:331682/76‑1                                                                                                                                                     ATCATGATCGATAATTACATCGCGTACACCGGCAGCATGAATATGGTCGACCCTCGCTACTTCAAACAAGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/1305558‑1305487 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA  <  REL606/2775877‑2775803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.