| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,404 | T→G | Q52H (CAA→CAC) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,404 | 0 | T | G | 97.5% | 162.1 / ‑4.1 | 40 | Q52H (CAA→CAC) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (24/15); total (25/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01 | |||||||||||
GACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGC > REL606/4202332‑4202476 | gACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCgtgt > 2:851067/1‑76 (MQ=255) cTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGAGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTgc > 2:766365/1‑76 (MQ=255) tGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTgcg > 1:1018363/1‑76 (MQ=255) gACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTgcgc > 1:1151007/1‑76 (MQ=255) cGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCa < 2:708233/76‑1 (MQ=255) cGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCa > 2:294307/1‑76 (MQ=255) gCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCaa < 2:633160/76‑2 (MQ=255) gCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGGGGGACGTGGAATTGGGTAACCCGGCGTGTTGCGcccg > 2:935142/1‑74 (MQ=255) aaaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCg > 1:1019150/1‑76 (MQ=255) aaCCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGt < 2:1000910/76‑1 (MQ=255) ctgTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGcc > 2:1445482/1‑76 (MQ=255) ctgTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGcc > 2:486194/1‑76 (MQ=255) ctgTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGAGTGGTGCGCCAGTCCCGTGAGTGcc > 2:796339/1‑76 (MQ=255) tgTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCca < 2:578653/76‑1 (MQ=255) gTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCcac > 1:566000/1‑76 (MQ=255) tGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCacac > 1:706937/1‑76 (MQ=255) gCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACt < 1:819223/76‑1 (MQ=255) cATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTg < 2:153276/76‑1 (MQ=255) aTTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGCGAGTGCCACACTGc > 2:373167/1‑76 (MQ=255) tgtgGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGTGTGCCACACTGCCa < 1:793527/76‑1 (MQ=255) gtgGTTAGCAGGCGGTGGGTCGTGGAAGTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCAt < 1:1337787/76‑1 (MQ=255) tgGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCAtt > 1:149715/1‑76 (MQ=255) aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCgg > 1:587388/1‑76 (MQ=255) aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTCCGCAAGTTCCGCGAGTCCCAAACTGCCATCGGATCCga > 1:1428849/1‑75 (MQ=255) gTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATc > 1:407739/1‑76 (MQ=255) naaTTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTa > 1:1135028/2‑76 (MQ=255) aaTTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTaa > 2:1141156/1‑76 (MQ=255) aTTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAAt < 1:29896/76‑1 (MQ=255) tGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAAttt < 1:739574/76‑1 (MQ=255) tGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAAttt < 2:917688/76‑1 (MQ=255) ggTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCa > 2:1991/1‑76 (MQ=255) ggTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCa > 1:413169/1‑76 (MQ=255) tAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAgg < 1:720276/76‑1 (MQ=255) aaCCCGGCGTGTTNCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGc > 1:1004972/1‑76 (MQ=255) aaCCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGc > 1:870868/1‑76 (MQ=255) aaCCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTGCAGGc > 2:1477921/1‑76 (MQ=255) cccGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCa < 1:554190/76‑1 (MQ=255) cGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCTCTCCAGTAATTTCAGGCCAcg < 2:413919/76‑1 (MQ=255) cGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCAcg > 2:1106222/1‑76 (MQ=255) cGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCAcg < 1:581788/76‑1 (MQ=255) cGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCAcg > 1:517230/1‑76 (MQ=255) ggCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCAcgc > 1:278083/1‑76 (MQ=255) | GACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGC > REL606/4202332‑4202476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |