| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,404,030 | T→A | F13I (TTC→ATC) | rpsR → | 30S ribosomal protein S18 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,404,030 | 0 | T | A | 98.6% | 273.8 / ‑3.1 | 71 | F13I (TTC→ATC) | rpsR | 30S ribosomal protein S18 |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (36/34); total (37/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01 | |||||||||||
GAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCG > REL606/4403957‑4404102 | gAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATc < 1:39883/76‑1 (MQ=255)gAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATc > 1:603880/1‑76 (MQ=255) aGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCa > 2:473238/1‑76 (MQ=255) gCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCAc < 1:491897/76‑1 (MQ=255) cAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCAcc > 2:581200/1‑76 (MQ=255) cAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCAcc > 1:734763/1‑76 (MQ=255) aGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACcg > 1:1231420/1‑76 (MQ=255) gATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACcgc < 2:897981/76‑1 (MQ=255) ttgaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCgg > 2:1118333/1‑76 (MQ=255) ttgaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCgg > 1:1481834/1‑76 (MQ=255) ttgaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCgg > 1:110071/1‑76 (MQ=255) ttgaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCgg > 1:992651/1‑76 (MQ=255) ttgaattgaTAGATGCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCgg < 1:138196/76‑1 (MQ=255) tgaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGa > 1:753380/1‑76 (MQ=255) tgaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGa > 1:373358/1‑76 (MQ=255) agaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGa > 1:824880/2‑76 (MQ=255) gaattgaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGaa > 1:672366/1‑76 (MQ=255) gaTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGt < 1:93635/76‑1 (MQ=255) aTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGtt > 2:1443480/1‑76 (MQ=255) aTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGtt < 1:390903/76‑1 (MQ=255) gATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCaa < 1:56016/76‑1 (MQ=255) gATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCaa > 2:721565/1‑76 (MQ=255) gATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCaa < 2:94617/76‑1 (MQ=255) aTTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAag < 2:1069825/76‑1 (MQ=255) aTTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAag > 1:948311/1‑76 (MQ=255) aTTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAag > 1:258196/1‑76 (MQ=255) aTTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAag < 1:1282521/76‑1 (MQ=255) aTTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAag < 2:894258/76‑1 (MQ=255) cTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAgaga < 2:1208829/76‑1 (MQ=255) gagaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCg < 1:284785/76‑1 (MQ=255) agaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGa < 1:369745/76‑1 (MQ=255) aCTAGCCATATGGCACGTTATTTCCGTCGTTGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACt > 1:912656/1‑76 (MQ=255) aCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACt < 2:436052/76‑1 (MQ=255) aCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACt < 1:608684/76‑1 (MQ=255) aCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACt < 2:1132839/76‑1 (MQ=255) cTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACta > 2:330867/1‑76 (MQ=255) cTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACta < 1:1097300/76‑1 (MQ=255) tAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACtat > 2:785336/1‑76 (MQ=255) tAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACtat > 2:114569/1‑76 (MQ=255) aGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACtata > 1:682833/1‑76 (MQ=255) aGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACtata > 1:1237564/1‑76 (MQ=255) gCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATaa < 2:1427227/76‑1 (MQ=255) ccATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATaaa < 2:1051327/76‑1 (MQ=255) cATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAg > 1:1445644/1‑76 (MQ=255) cATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAg > 1:340728/1‑76 (MQ=255) atGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGata < 1:1082029/76‑1 (MQ=255) tGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGttat < 2:982411/76‑1 (MQ=255) gCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCg > 1:90491/1‑76 (MQ=255) aCGTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCt < 1:623090/76‑1 (MQ=255) ctttATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTa > 1:1083278/3‑76 (MQ=255) gTTATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTAc < 2:505587/76‑1 (MQ=255) ttATTTCCGTCGTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACg < 2:570778/76‑1 (MQ=255) ccGTCTTCGCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaa < 2:887768/76‑1 (MQ=255) ccGTCGTCGCAAGTTCTGCCGTATCTCCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaa < 1:1335067/76‑1 (MQ=255) cgtcgCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACt > 1:212445/1‑76 (MQ=255) cgtcgCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACt < 1:715918/76‑1 (MQ=255) cgCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACa < 2:1095714/76‑1 (MQ=255) gCAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACAt < 1:393068/76‑1 (MQ=255) gCAAGTTCTGCCGATTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACAt > 1:997055/1‑76 (MQ=255) cAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATc < 2:569261/76‑1 (MQ=255) cAAGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATc > 1:356738/1‑76 (MQ=255) aaGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCa < 1:128059/76‑1 (MQ=255) aGTTCTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCAc > 1:566970/1‑76 (MQ=255) cTGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaa > 1:917148/1‑76 (MQ=255) tGCTGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaaa < 2:213727/76‑1 (MQ=255) tGCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaaa > 2:956271/1‑76 (MQ=255) gCCGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAg < 2:1481326/76‑1 (MQ=255) ccGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGc > 2:416713/1‑76 (MQ=255) cGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCg > 2:1189482/1‑76 (MQ=255) cGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCg < 1:742390/76‑1 (MQ=255) cGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCg < 2:74372/76‑1 (MQ=255) cGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCg > 1:916965/1‑76 (MQ=255) cGTATCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCg > 2:1211091/1‑76 (MQ=255) | GAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCG > REL606/4403957‑4404102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |