| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,645,442 | G→A | S106S (AGC→AGT) | mlc ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,645,442 | 0 | G | A | 98.4% | 230.9 / ‑4.0 | 61 | S106S (AGC→AGT) | mlc | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (33/27); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01 | |||||||||||
GGGAAATAATACGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTGCTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAGGCTTCA > REL606/1645369‑1645514 | gggAAATAATACGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTtact < 2:844628/76‑1 (MQ=255)gggAAATAATACGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTtact > 1:186217/1‑76 (MQ=255) gAAATAATACGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTtactgc > 1:905204/1‑76 (MQ=255) aataatACGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTc > 2:518767/1‑76 (MQ=255) ataataCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCa < 1:740086/76‑1 (MQ=255) ataataCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCa > 1:1152985/1‑76 (MQ=255) ataataCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCa > 1:702509/1‑76 (MQ=255) aaaataCAATCAATCAATGACAAGTCGTCTTAAAACACCAGTTCCTGCGACTCTGCCACCACCAGTTTACTGCTCa < 2:738457/74‑1 (MQ=255) taataCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAg > 2:1386349/1‑76 (MQ=255) aataCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGa > 1:1436890/1‑76 (MQ=255) aataCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGa > 2:310568/1‑76 (MQ=255) aataCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGa > 2:486419/1‑76 (MQ=255) ataCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGAt > 1:1012170/1‑76 (MQ=255) ataCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGAt > 1:264023/1‑76 (MQ=255) taCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATc < 1:365174/76‑1 (MQ=255) taCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATc > 1:788911/1‑76 (MQ=255) aCGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATcg < 2:266967/76‑1 (MQ=255) cGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCAGCACCAGTTTACTGCTCAGATcgc > 2:737793/1‑76 (MQ=255) gATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATcgcg < 1:343128/76‑1 (MQ=255) gATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATcgcg > 2:659709/1‑76 (MQ=255) aTCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCgcgc > 1:313837/1‑76 (MQ=255) aTCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCgcgc < 2:778683/76‑1 (MQ=255) cccAGCAATGGCAAGTCATCTTTTAACGCCAATTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCa < 2:895298/75‑1 (MQ=255) ccAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCag < 2:1054773/76‑1 (MQ=255) cAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCaga > 2:548805/1‑76 (MQ=255) gCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTGCTGCTCAGATCGCGCAGAGc < 2:152625/76‑1 (MQ=255) cAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCa > 2:1360743/1‑76 (MQ=255) cAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCa > 2:299710/1‑76 (MQ=255) cAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCa < 2:180994/76‑1 (MQ=255) cAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCa > 2:1182751/1‑76 (MQ=255) aaTGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCaa > 2:94063/1‑76 (MQ=255) gCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGaa < 2:571973/76‑1 (MQ=255) cAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGaaa < 1:318048/76‑1 (MQ=255) aaGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGaaaa < 2:1297455/76‑1 (MQ=255) aTCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTcc > 2:562635/1‑76 (MQ=255) ttttAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCcg < 1:61348/76‑1 (MQ=255) ttAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCcgcg > 1:1078832/1‑76 (MQ=255) ttAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCcgcg < 1:970562/76‑1 (MQ=255) ttAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCcgcg > 1:599941/1‑76 (MQ=255) aaCGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGAc > 1:454536/1‑76 (MQ=255) aCGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACt < 1:269053/76‑1 (MQ=255) cGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACtn < 1:928936/76‑2 (MQ=255) cGCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTa < 1:1414481/76‑1 (MQ=255) gCCAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTaa < 1:1159107/76‑1 (MQ=255) ccAGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAAt > 2:490595/1‑76 (MQ=255) aGTTCCTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATgc > 1:355215/1‑76 (MQ=255) ccTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTCATGCGCag > 2:503292/1‑76 (MQ=255) ccTGCGACTCTTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCag > 2:329122/1‑76 (MQ=255) ctctTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGa < 1:1447029/76‑1 (MQ=255) tctTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGAt < 1:229339/76‑1 (MQ=255) ctTCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATa > 2:143868/1‑76 (MQ=255) ttCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAg > 2:600308/1‑76 (MQ=255) tCCACCACCAGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGt > 2:922717/1‑76 (MQ=255) ccaccaccaGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTg < 2:783381/76‑1 (MQ=255) caccaccaGTTTGCTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGc < 2:1198360/76‑1 (MQ=255) cctccaGTATACTGCTCAGACCGCGCAGAGCAAGGAAAATCCCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCa < 1:480297/73‑1 (MQ=255) ccaccaGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGCCTACTGCGCAGCGAAACATAGTGCCa > 2:1383197/1‑76 (MQ=255) ccaccaGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCa < 2:838590/76‑1 (MQ=255) caccaGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAg > 1:1250130/1‑76 (MQ=255) accaGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAgg < 1:1448690/76‑1 (MQ=255) accaGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAgg < 1:1247112/76‑1 (MQ=255) ccaGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAGGc > 1:402382/1‑76 (MQ=255) aGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAGGCtt < 2:970031/76‑1 (MQ=255) aGTTTACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAGGCtt < 1:1214020/76‑1 (MQ=255) tttACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAGGCTTCa < 1:1225586/76‑1 (MQ=255) tttACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAGGCTTCa < 1:113513/76‑1 (MQ=255) tttACTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAGGCTTCa > 2:1091904/1‑76 (MQ=255) | GGGAAATAATACGATCCAGCAATGGCAAGTCATCTTTTAACGCCAGTTCCTGCGACTCTTCCACCACCAGTTTGCTGCTCAGATCGCGCAGAGCAAGGAAAATCTCCCCGCGACTAATGCGCAGAGAAAGATAGTGCCAGGCTTCA > REL606/1645369‑1645514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |