Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 422,600 (G)7→8 intergenic (‑287/+175) cyoA ← / ← ampG cytochrome o ubiquinol oxidase subunit II/muropeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606422,5931.G91.7% 185.9 / 10.9 48intergenic (‑280/+182)cyoA/ampGcytochrome o ubiquinol oxidase subunit II/muropeptide transporter
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base G (25/19);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

GACGGGCAATTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCAATAATTTCC  >  REL606/422518‑422668
                                                                             |                                                                           
gACGGGCAATTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATtgggt                                                                              <  1:1749215/76‑1 (MQ=255)
 aCGGGCAATTGGTATAACCACTCTGGAATAAATCAATTGTTTAACAAATAATTATCATTATGGTGACAATtgggtg                                                                            <  2:290797/76‑1 (MQ=255)
 aCGGGCAATTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATtgggtg                                                                            <  2:188914/76‑1 (MQ=255)
    ggCAATTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTgggg                                                                         <  2:1609427/76‑1 (MQ=255)
       aaatGGTAAAAACCAACTTGAAAAAAACAAATGTTTAAAAAATAATTATCATTATGGTGACAATTGGGTggggggg                                                                      <  2:1504584/73‑1 (MQ=255)
       aaTTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTggggggg                                                                      <  1:54736/76‑1 (MQ=255)
          tGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGgaa                                                                   <  1:556677/76‑1 (MQ=255)
            gTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGgaaaa                                                                 <  2:1616286/76‑2 (MQ=255)
                aaCCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATaaa                                                              >  2:451843/1‑76 (MQ=255)
                aaCCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATaaa                                                              >  1:114179/1‑76 (MQ=255)
                  ccAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGa                                                            <  2:1375957/76‑1 (MQ=255)
                  ccAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGa                                                            >  1:762335/1‑76 (MQ=255)
                     aTCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTc                                                         >  1:1392863/1‑76 (MQ=255)
                      tCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCa                                                        <  1:911008/76‑1 (MQ=255)
                       cTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCAt                                                       >  1:514123/1‑76 (MQ=255)
                         ggAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTa                                                     >  2:1611506/1‑76 (MQ=255)
                         ggAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTa                                                     <  2:1092309/76‑1 (MQ=255)
                          gAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAg                                                    <  2:660497/76‑1 (MQ=255)
                             tAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTg                                                 <  1:350321/76‑1 (MQ=255)
                             tAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTg                                                 >  2:1739132/1‑76 (MQ=255)
                              aaaaCAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGa                                                >  1:1085844/1‑76 (MQ=255)
                                   aaTGGTTAAACAATTATTTATATTAATGTGACTAGTTGGGTGGGGGGAAAATAAAGACTCATTAGCTGAATCgtgt                                         <  1:626931/76‑1 (MQ=255)
                                    aTTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCgtg                                          <  1:578945/76‑1 (MQ=255)
                                     ttGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCgtgt                                         >  1:536389/1‑76 (MQ=255)
                                     ttGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCgtgt                                         <  2:770205/76‑1 (MQ=255)
                                      tGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTa                                        <  1:1167549/76‑1 (MQ=255)
                                      tGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTa                                        >  2:1062490/1‑76 (MQ=255)
                                       gTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTaa                                       >  1:406950/1‑76 (MQ=255)
                                       gTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTaa                                       >  1:341938/1‑76 (MQ=255)
                                       gTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTaa                                       <  2:1458183/76‑1 (MQ=255)
                                        tttAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAg                                      >  1:1380766/1‑76 (MQ=255)
                                        tttAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAg                                      <  1:1787292/76‑1 (MQ=255)
                                         ttAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGa                                     <  1:569549/76‑1 (MQ=255)
                                         ttAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGa                                     >  1:1683809/1‑76 (MQ=255)
                                         ttAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGa                                     >  2:147071/1‑76 (MQ=255)
                                          tAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGGAAATAAAGACTCATTAGCTGAATCGTGTAAGaa                                    >  1:343154/1‑76 (MQ=255)
                                          tAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGaa                                    >  1:252950/1‑76 (MQ=255)
                                               aataatTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAAt                               <  1:1731240/76‑1 (MQ=255)
                                                 taatTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAg                             >  1:1719405/1‑76 (MQ=255)
                                                   atTATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGaa                           >  1:682789/1‑76 (MQ=255)
                                                    ttATCATTATGGTAACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGACAAAATAGAAt                          >  1:1204803/1‑76 (MQ=255)
                                                     tATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAAtt                         >  1:1030004/1‑76 (MQ=255)
                                                     tATCATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAAtt                         >  1:125127/1‑76 (MQ=255)
                                                     tATCATTATGGTGACAATTGGGTGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTc                        <  2:939912/76‑1 (MQ=255)
                                                        cATTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCAt                      <  2:999057/76‑1 (MQ=255)
                                                         aTTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCGTTAGCTGAATCGTGTAAGAAAAAATAGAATTCaaa                     <  2:1627092/76‑3 (MQ=255)
                                                         aTTATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATa                     <  2:564519/76‑1 (MQ=255)
                                                         aTTATGGTGACAATTGGGTGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATaa                    >  1:1577138/1‑76 (MQ=255)
                                                           tATGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATaaa                   >  2:1025060/1‑76 (MQ=255)
                                                            aTGGTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGCATCGTGTAAGAAAAAATAGAATTCATAAAt                  >  2:977434/1‑76 (MQ=255)
                                                              ggTGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTg                <  2:255767/76‑1 (MQ=255)
                                                                tGACAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCg              >  1:671386/1‑76 (MQ=255)
                                                                   cAATTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCa           <  1:1615956/76‑1 (MQ=255)
                                                                      aTTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCaat         <  1:1802286/76‑1 (MQ=255)
                                                                      aTTGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCaat         <  1:1185431/76‑1 (MQ=255)
                                                                       ttGGGTGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCaata        <  1:1724880/76‑1 (MQ=255)
                                                                        tgggtgggGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCaataa       >  1:140789/1‑76 (MQ=255)
                                                                          ggtgggGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCAATAAttt    <  2:1631451/76‑1 (MQ=255)
                                                                            tgggAGGGAAAATACAGAATCATAAGATGAATAGTGTAAGAAAAAATAGAATTTATAAATTGCGTCAATACTTTcc  >  2:1361675/1‑76 (MQ=255)
                                                                             |                                                                           
GACGGGCAATTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGTGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCAATAATTTCC  >  REL606/422518‑422668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: