Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 398,585 C→A R220S (CGT→AGT)  secF → protein export protein SecF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606398,5850CA95.2% 143.9 / 0.5 42R220S (CGT→AGT) secFprotein export protein SecF
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (25/15);  total (25/17)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

TGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGA  >  REL606/398512‑398657
                                                                         |                                                                        
tgacctgaccATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGt                                                                        >  1:469891/1‑76 (MQ=255)
tgacctgaccATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGt                                                                        <  1:1491542/76‑1 (MQ=255)
 gacctgaccATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTa                                                                       <  1:560915/76‑1 (MQ=255)
 gacctgaccATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTa                                                                       <  1:396199/76‑1 (MQ=255)
    ctgaccATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTc                                                                    <  1:339832/76‑1 (MQ=255)
      gaccATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGt                                                                  >  2:1382289/1‑76 (MQ=255)
         cATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGaa                                                               >  2:379344/1‑76 (MQ=255)
          aTTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGaaa                                                              >  1:1795030/1‑76 (MQ=255)
          aTTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGaaa                                                              >  1:85787/1‑76 (MQ=255)
             gtgGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACt                                                           <  1:1440411/76‑1 (MQ=255)
              tgGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACtt                                                          <  1:301598/76‑1 (MQ=255)
               ggCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGGAAACTTc                                                         <  1:1170573/76‑1 (MQ=255)
                  aTCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGc                                                      >  1:593572/1‑76 (MQ=255)
                  aTCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGc                                                      >  1:817695/1‑76 (MQ=255)
                   tCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCa                                                     >  1:232884/1‑76 (MQ=255)
                   tCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGGGAAAACTTCCGCa                                                     >  1:1729465/1‑76 (MQ=255)
                    cGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCaa                                                    >  1:1769155/1‑76 (MQ=255)
                             cggttatcggttaCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTcg                                           <  2:346639/76‑1 (MQ=255)
                               gttatcggttaCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTcgcg                                         >  2:465020/1‑76 (MQ=255)
                                ttatcggttaCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCgg                                        <  1:1118842/76‑1 (MQ=255)
                                 tatcggttaCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGt                                       >  2:1494905/1‑76 (MQ=255)
                                 tatcggttaCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGt                                       <  2:1471884/76‑1 (MQ=255)
                                  atcggttaCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTa                                      >  2:849214/1‑76 (MQ=255)
                                   tcggttaCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTAc                                     >  2:1375920/1‑76 (MQ=255)
                                    cggttaCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACg                                    >  1:359515/1‑76 (MQ=255)
                                    cggttaCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACg                                    >  1:99064/1‑76 (MQ=255)
                                        ttcTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCtt                                <  2:1409657/74‑1 (MQ=255)
                                        taCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCtt                                >  2:1366427/1‑76 (MQ=255)
                                        taCTCGCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCtt                                <  1:269499/76‑1 (MQ=255)
                                           tCGCTTAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACg                             <  1:1049956/76‑1 (MQ=255)
                                            cGCTTAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGa                            <  1:761724/76‑1 (MQ=255)
                                             gCTTAACGACAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGaa                           >  1:1549582/1‑76 (MQ=255)
                                                      cAGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAAc                  >  1:625600/1‑76 (MQ=255)
                                                       aGTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACg                 <  1:1200605/76‑1 (MQ=255)
                                                        gTATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACgt                <  1:1652536/76‑1 (MQ=255)
                                                         tATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACgtg               >  2:1423686/1‑76 (MQ=255)
                                                         tATCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACgtg               <  2:568176/76‑1 (MQ=255)
                                                          aTCGTGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACgtgt              >  1:1043234/1‑76 (MQ=255)
                                                              tgctATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGCAATCTTTAACGTGTCCtt          >  2:123845/4‑76 (MQ=255)
                                                              tGGTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCtt          <  2:1386459/76‑1 (MQ=255)
                                                                gTATCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGa        >  2:947730/1‑76 (MQ=255)
                                                                  aTCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGAcc      >  2:1484921/1‑76 (MQ=255)
                                                                  aTCGGACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGAcc      >  2:1533952/1‑76 (MQ=255)
                                                                      gACAGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGa  >  1:54195/1‑76 (MQ=255)
                                                                         |                                                                        
TGACCTGACCATTGTGGCATCGTTGATGTCGGTTATCGGTTACTCGCTTAACGACAGTATCGTGGTATCGGACCGTATTCGTGAAAACTTCCGCAAGATCCGTCGCGGTACGCCTTACGAAATCTTTAACGTGTCCTTGACCCAGA  >  REL606/398512‑398657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: