| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,470 | A→C | T539P (ACC→CCC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,470 | 0 | A | C | 94.7% | 141.7 / ‑3.2 | 38 | T539P (ACC→CCC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (25/11); total (26/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAA > REL606/4162397‑4162542 | tATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGccc < 1:1428868/76‑1 (MQ=255) tGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCg < 2:496375/76‑1 (MQ=255) ggACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGt > 1:71950/1‑76 (MQ=255) aGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGGACg > 1:1166000/1‑76 (MQ=255) gAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCACCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGt < 2:1652776/76‑1 (MQ=255) aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCCTCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTg < 2:1651210/76‑1 (MQ=255) acaacCCTCTGTCTGAGAATACGCACAAACGTCATATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGc < 2:1164173/76‑1 (MQ=255) naacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCa > 1:1691799/2‑76 (MQ=255) caacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCa > 1:431642/1‑76 (MQ=255) aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGACCGTGCAg > 2:1247125/1‑76 (MQ=255) cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGc > 2:34646/1‑76 (MQ=255) ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCt > 2:449538/1‑76 (MQ=255) cGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCtt > 2:207409/1‑76 (MQ=255) cGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCtt < 1:150060/76‑1 (MQ=255) gCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTc > 1:96606/1‑76 (MQ=255) cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCg > 1:1422771/1‑76 (MQ=255) gTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGaa > 2:1440245/1‑76 (MQ=255) gTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGaa > 2:523385/1‑76 (MQ=255) tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGGGCAGGGTTCGacg > 1:911540/1‑74 (MQ=255) tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGtt < 1:805636/76‑1 (MQ=255) gagaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTc > 2:111225/1‑76 (MQ=255) gagaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTc > 1:645334/1‑76 (MQ=255) ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCgaga < 1:779736/76‑1 (MQ=255) ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGGCTGCGCCGTGAGAGATCAAGCTTAGAAGATCgaga > 2:1025206/1‑76 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACg > 2:557230/1‑76 (MQ=255) gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTa < 1:656334/76‑1 (MQ=255) cgtcgtATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGa > 2:916373/1‑76 (MQ=255) gtcgtATCTCCGCACTCGGCCCAGGCTGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGAc > 1:146753/1‑76 (MQ=255) tcgtATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACt > 1:427178/1‑76 (MQ=255) aTCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACt > 2:1036994/1‑76 (MQ=255) tcCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACg > 2:1521908/1‑76 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTAcag > 2:1283379/1‑74 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACgg > 1:584965/1‑76 (MQ=255) gCACTCGGCCCAGGGGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTc < 1:448260/76‑1 (MQ=255) gCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTc > 2:979246/1‑76 (MQ=255) cACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTcg < 2:1239043/76‑1 (MQ=255) cGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTa < 2:1197538/76‑1 (MQ=255) ggCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTAt > 2:1300691/1‑76 (MQ=255) cGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATc > 1:1629399/1‑76 (MQ=255) cGGTCTGCCCCGTGAACGTGCAGCCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATc > 1:941961/1‑76 (MQ=255) ggTCTGACCCGTGAACGTACAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCg < 2:75205/76‑1 (MQ=255) tCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaa < 2:431642/76‑1 (MQ=255) cTGCCCCGTGAACGTGCAGGCTTCGAAGATCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaaa > 1:1428693/1‑76 (MQ=255) cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaaa > 2:1770415/1‑76 (MQ=255) | TATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAA > REL606/4162397‑4162542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |