| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,163,089 | A→C | E745A (GAA→GCA) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,163,089 | 0 | A | C | 97.2% | 295.6 / ‑2.4 | 72 | E745A (GAA→GCA) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (34/36); total (35/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCC > REL606/4163016‑4163161 | ggtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAg > 1:1108152/1‑76 (MQ=255) gtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGc < 2:1220764/76‑1 (MQ=255) ggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGcagcag > 1:1278209/1‑76 (MQ=255) tCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTAt < 1:1424474/76‑1 (MQ=255) tCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTAt < 2:1553319/76‑1 (MQ=255) gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCg < 2:1800490/76‑1 (MQ=255) gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCg < 2:1354081/76‑1 (MQ=255) gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCg < 1:375004/76‑1 (MQ=255) gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGAGCAGCAGGTATCg > 1:1746786/1‑76 (MQ=255) ttCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGa > 2:1220189/1‑76 (MQ=255) ttCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGa > 1:1129546/1‑76 (MQ=255) tCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGAc > 2:1635974/1‑76 (MQ=255) cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGGGCAGCAGGTATCGACa > 1:428472/1‑76 (MQ=255) gTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATc < 1:669146/76‑1 (MQ=255) tACGTGGATCCTTCCCGTATCGTTAGCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATcc < 1:1249731/76‑2 (MQ=255) aCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTa < 1:1155965/76‑1 (MQ=255) aCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTa > 1:319435/1‑76 (MQ=255) gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACa < 2:857091/76‑1 (MQ=255) gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACa > 1:1477348/1‑76 (MQ=255) tGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACaa < 2:853144/76‑1 (MQ=255) ggATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAAc > 2:389047/1‑76 (MQ=255) tGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTg < 1:1334299/76‑1 (MQ=255) tGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTg < 1:1103443/76‑1 (MQ=255) tGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTg < 1:632194/76‑1 (MQ=255) tGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGAATCCGGGTGCAGCAGGAATCGACATCTACAACCTg < 2:751985/76‑1 (MQ=255) gCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGa > 1:821107/1‑76 (MQ=255) ttCCCGTATCGTTATCAAATTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGAcc < 1:392566/76‑1 (MQ=255) tCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCa > 1:1517548/1‑76 (MQ=255) cGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAAt > 1:1718105/1‑76 (MQ=255) gTATCGTTATCAAATTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATa < 1:392776/76‑1 (MQ=255) gTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACAAAATa < 2:868970/76‑1 (MQ=255) tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATac > 2:667826/1‑76 (MQ=255) aTCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATaca > 1:1682998/1‑76 (MQ=255) tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGCATCGACATCTACAACCCGACCAAATACAcccct > 2:310274/1‑74 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt > 1:1805366/1‑76 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt > 1:439170/1‑76 (MQ=255) aaaGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACTCCCGTTCTa < 1:1325343/76‑1 (MQ=255) aaaGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCtt < 1:1005811/76‑2 (MQ=255) aaaGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTa < 2:1441304/76‑1 (MQ=255) aaaGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTa > 2:564826/1‑76 (MQ=255) aGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAc > 1:1767606/1‑76 (MQ=255) aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAc > 2:1776619/1‑76 (MQ=255) gTTAACGAATGCGAGAGGTATCCGTGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACGCCCGTTCTAAcc < 2:1260339/76‑1 (MQ=255) gTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAcc < 2:1374332/76‑1 (MQ=255) gTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAcc < 1:1617036/76‑1 (MQ=255) ttAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCa > 1:1001387/1‑76 (MQ=255) ttAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACAAAATACACCCGTCCTAACCa > 1:758385/1‑76 (MQ=255) tAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAg > 1:1243929/1‑76 (MQ=255) aaCGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGa > 1:1444928/1‑76 (MQ=255) cGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAac < 1:298340/76‑1 (MQ=255) gAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAaca > 1:1757225/1‑76 (MQ=255) gAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAaca < 1:1756411/76‑1 (MQ=255) gAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAaca > 1:1206102/1‑76 (MQ=255) aGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACAcc < 1:1170897/76‑1 (MQ=255) aCGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTg < 1:980147/76‑1 (MQ=255) aCGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTg > 1:275769/1‑76 (MQ=255) gaTGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATc < 2:1729839/76‑1 (MQ=255) aTGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCa > 2:97806/1‑76 (MQ=255) aTGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCa < 1:624662/76‑1 (MQ=255) gTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAc > 1:413063/1‑76 (MQ=255) tATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACCCCCGTTCTAACCAGAACACCTGTATCaaac < 1:830734/76‑3 (MQ=255) tATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc > 2:1670030/1‑76 (MQ=255) tATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc < 1:750558/76‑1 (MQ=255) tATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc > 1:1387126/1‑76 (MQ=255) aTCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa > 2:1479773/1‑76 (MQ=255) aTCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa > 1:821371/1‑76 (MQ=255) aTCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa < 2:567120/76‑1 (MQ=255) aTCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa < 1:1198877/76‑1 (MQ=255) cGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGAt > 2:263330/1‑76 (MQ=255) cGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGAt < 2:80369/76‑1 (MQ=255) gggTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATg < 2:1032742/76‑1 (MQ=255) gggTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATg < 1:1294723/76‑1 (MQ=255) ggTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGc > 2:1496725/1‑76 (MQ=255) gTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGcc < 2:570070/76‑1 (MQ=255) | GGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCC > REL606/4163016‑4163161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |