| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,462 | A→G | L33S (TTA→TCA) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,462 | 0 | A | G | 94.4% | 128.1 / ‑1.0 | 36 | L33S (TTA→TCA) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (15/19); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
TGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC > REL606/4202391‑4202534 | tGGGTAACCCGGCTTGTTGCGCCAGTGCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCTCTCCAGTAAttt < 2:918089/76‑1 (MQ=255) gggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTc < 2:919877/76‑1 (MQ=255) gggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGCGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTc > 1:1366244/1‑76 (MQ=255) gcgTAACCCGGCTTGTTGCGCCAGTTCCGTGACTGCCACACTGCCATTGGATTCGCCAATCCACTCCAGTGATTTc < 2:1601407/74‑1 (MQ=255) ggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCa < 2:1191951/76‑1 (MQ=255) gTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAg > 1:1040790/1‑76 (MQ=255) aCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGcc > 1:1109984/1‑76 (MQ=255) cTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGt > 1:849131/1‑76 (MQ=255) tgttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTa > 1:1104325/1‑76 (MQ=255) gttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTaa < 1:579930/76‑1 (MQ=255) tgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAg < 1:1468223/76‑1 (MQ=255) gcgcCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGa < 1:1146750/76‑1 (MQ=255) aGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGaa < 1:15221/76‑1 (MQ=255) tCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCt < 1:1035176/76‑1 (MQ=255) ccGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTg < 2:1189962/76‑1 (MQ=255) gTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTc < 1:1019949/76‑1 (MQ=255) gAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCa < 1:385234/76‑1 (MQ=255) tGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTc > 2:1714486/1‑76 (MQ=255) acacTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTg < 2:571231/76‑1 (MQ=255) acTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGt > 1:171130/1‑76 (MQ=255) acTGCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGCCGCTGGt > 1:846950/1‑76 (MQ=255) gCCATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCg > 1:204816/1‑76 (MQ=255) ccATTGGATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCgg > 1:1277156/1‑76 (MQ=255) ggATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCa < 2:1768356/76‑1 (MQ=255) gATTCGGCAATCCTCTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCaa < 2:1707548/76‑1 (MQ=255) gATTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCaa < 1:449414/76‑1 (MQ=255) aTTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAAc > 2:406968/1‑76 (MQ=255) aTTCGGCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGCGGCaag > 2:250515/1‑75 (MQ=255) ggCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAAcggcg < 1:304478/76‑1 (MQ=255) ggCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAAcggcg < 2:550460/76‑1 (MQ=255) gCAATCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAAcggcgg > 2:1664634/1‑76 (MQ=255) aTCCACTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCggggt > 1:384454/1‑74 (MQ=255) cTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTg < 2:986012/76‑1 (MQ=255) cTCCAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTg < 2:659036/76‑1 (MQ=255) ccAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGcc > 2:66096/1‑76 (MQ=255) ccAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGcc > 1:219127/1‑76 (MQ=255) cAGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCcg < 1:445249/76‑1 (MQ=255) aGTGATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCcgc < 2:708596/76‑1 (MQ=255) | TGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC > REL606/4202391‑4202534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |