| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,250,032 | G→A | S406F (TCT→TTT) | infB ← | translation initiation factor IF‑2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,250,032 | 0 | G | A | 98.0% | 188.6 / NA | 50 | S406F (TCT→TTT) | infB | translation initiation factor IF‑2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (24/25); minor base C (1/0); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGT > REL606/3249964‑3250104 | cAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGtttt < 1:1256695/76‑1 (MQ=255) aaTGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTa < 2:1694731/76‑1 (MQ=255) tgCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGt > 1:1258720/1‑76 (MQ=255) gCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTg > 1:239675/1‑76 (MQ=255) tGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGt > 2:69987/1‑76 (MQ=255) ggTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCa < 1:719500/76‑1 (MQ=255) ggTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCa < 1:635594/76‑1 (MQ=255) gTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCaa < 2:157657/76‑1 (MQ=255) tAATGCCGCCCGCTTCGCCGGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTCCCGTGGTCAAc < 2:1404235/76‑1 (MQ=255) aTGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGt < 2:1025056/76‑1 (MQ=255) tGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTg < 2:776982/76‑1 (MQ=255) tGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTg < 2:443224/76‑1 (MQ=255) gcccgcTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGAccc < 1:860335/76‑1 (MQ=255) gcccgcTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGAccc > 2:353025/1‑76 (MQ=255) gcccgcTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGAccc > 2:101479/1‑76 (MQ=255) ccgcTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGACAGGTTTTACCGTGGTCAACGTGCCCCat > 1:1794939/1‑76 (MQ=255) ccacTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCat > 1:1794924/4‑76 (MQ=255) cgcTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCatg < 1:527789/76‑1 (MQ=255) gcTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCatga > 2:528373/1‑76 (MQ=255) gcTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCatga > 1:515642/1‑76 (MQ=255) cTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCatgat < 2:781208/76‑1 (MQ=255) ttCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCatgatg > 1:216572/1‑76 (MQ=255) cGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGt > 1:544359/1‑76 (MQ=255) gCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTc < 1:271613/76‑1 (MQ=255) gCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCAAGATGGTc < 1:651751/76‑1 (MQ=255) ccAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTca > 2:411997/1‑76 (MQ=255) cAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTcac < 1:4158/76‑1 (MQ=255) cAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTcac > 2:1615538/1‑76 (MQ=255) agagGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTcaca < 1:778654/76‑1 (MQ=255) agagGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTcaca > 1:216390/1‑76 (MQ=255) agagGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTcaca > 2:539453/1‑76 (MQ=255) gagGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACaa < 1:357341/76‑1 (MQ=255) agGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAAc > 2:718998/1‑76 (MQ=255) ggCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAAcc < 1:483570/76‑1 (MQ=255) ggCCACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAAcc < 1:1338405/76‑1 (MQ=255) cACTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGgc > 2:894195/1‑76 (MQ=255) cTTTCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGgcgc > 1:1792193/1‑76 (MQ=255) tttCGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGgcgcg < 2:1294179/76‑1 (MQ=255) cGTTGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGgcgcgcgc > 2:175783/1‑76 (MQ=255) ttGAACGAATGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCgg > 1:484422/1‑76 (MQ=255) aaCGAATGGAGTCCAGCAGAAAGGTTTTGCCGTGGTCAACGTGACCCAGGAGGGTCACAACCGGCGCGCGCGGTTc < 2:73709/76‑1 (MQ=255) aaTGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGcc > 2:147259/1‑76 (MQ=255) aaTGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGcc > 1:800265/1‑76 (MQ=255) aTGTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCg < 1:292975/76‑1 (MQ=255) gTAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCgca > 2:1225878/1‑76 (MQ=255) tAGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCgcag < 1:1662891/76‑1 (MQ=255) aGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCgcagc < 1:1540620/76‑1 (MQ=255) aGTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCgcagc > 2:700048/1‑76 (MQ=255) gTCCTGCAGAATGGTGTTACCGTGGTCAACGTGACCGATGATGGTCACAACCGGCGCGCGCGGTTCAGCCgcagca < 2:718347/76‑1 (MQ=38) gTCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCgcagca < 2:1633821/76‑1 (MQ=255) tCCAGCAGAAAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCAc > 2:1474148/1‑76 (MQ=255) agaaagGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCACCCCCACCACCCgggc > 1:496158/1‑73 (MQ=255) | CAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGT > REL606/3249964‑3250104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |