Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,489,216	C→T	intergenic (‑10/+7)	glpR ← / ← glpG	DNA‑binding transcriptional repressor/predicted intramembrane serine protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,489,216	0	C	T	100.0%	118.6 / NA	32	intergenic (‑10/+7)	glpR/glpG	DNA‑binding transcriptional repressor/predicted intramembrane serine protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/11);  total (21/11)

ACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  REL606/3489144‑3489288                                                                         |                                                                         aCTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTg                                                                       <  2:1452130/76‑1 (MQ=255)    gACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGaa                                                                    >  1:1445611/1‑76 (MQ=255)      cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAatt                                                                  >  1:1108608/1‑76 (MQ=255)       aTAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAatta                                                                 <  1:1034288/76‑1 (MQ=255)        tAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAattat                                                                >  2:68541/1‑76 (MQ=255)               gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTAttttcgtt                                                         >  2:1230364/1‑76 (MQ=255)                ctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTAttttcgttt                                                        >  2:595678/1‑76 (MQ=255)                  gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTAttttcgttttc                                                      >  2:1366747/1‑76 (MQ=255)                  gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTAttttcgttttc                                                      >  2:843695/1‑76 (MQ=255)                  gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTAttttcgttttc                                                      >  1:744827/1‑76 (MQ=255)                          aGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGCTTTCGCGCATTg                                              <  1:605969/76‑1 (MQ=255)                            ttCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAg                                            <  2:1547981/76‑1 (MQ=255)                            ttCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAg                                            <  2:1412191/76‑1 (MQ=255)                              cGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCg                                          <  2:317934/76‑1 (MQ=255)                               gATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGa                                         >  1:1648625/1‑76 (MQ=255)                                 taataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                       >  1:1811622/1‑76 (MQ=255)                                 taataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                       <  2:477284/76‑1 (MQ=255)                                        gTTGTGACGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                <  2:1487627/76‑1 (MQ=255)                                               cGTTGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc                         >  2:1786652/1‑76 (MQ=255)                                                 ttGTGTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc                       >  2:1472876/1‑76 (MQ=255)                                                  tgtgTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                      >  1:1312109/1‑76 (MQ=255)                                                  tgtgTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                      <  2:152321/76‑1 (MQ=255)                                                  tgtgTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                      <  2:1343905/76‑1 (MQ=255)                                                  tgtgTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                      >  1:62982/1‑76 (MQ=255)                                                  tgtgTTTGTTTCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                      >  1:545239/1‑76 (MQ=255)                                                       ttgtttCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc                 >  2:1612365/1‑76 (MQ=255)                                                        tgtttCATTTCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAccc                <  2:1270224/76‑1 (MQ=255)                                                               tttCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa         >  1:455848/1‑76 (MQ=255)                                                                ttCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTaa        >  1:1568995/1‑76 (MQ=255)                                                                 tCTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc       >  1:484586/1‑76 (MQ=255)                                                                  cTAAATCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc      >  1:1283033/1‑76 (MQ=255)                                                                    aaaTCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg    >  1:1374949/1‑76 (MQ=255)                                                                      aTCTCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc  >  1:878146/1‑76 (MQ=255)                                                                         |                                                                         ACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  REL606/3489144‑3489288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑