| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,131,458 | 0 | A | . | 95.5% | 195.3 / 1.4 | 44 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (34/8); total (35/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAC > REL606/2131387‑2131528 | gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:804976/1‑76 (MQ=255) aTTTGTTNCTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:1470308/1‑76 (MQ=255) tttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaa > 1:872902/1‑76 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 1:328784/1‑76 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:375068/1‑75 (MQ=255) ttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAACCgc > 1:919641/1‑75 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc < 2:902282/76‑1 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:874156/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc < 2:1084984/76‑1 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGACTccc > 2:75829/1‑76 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 1:745931/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 1:1055807/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 1:246672/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:186604/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 2:849386/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa < 2:1609496/76‑1 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa < 2:1581263/76‑1 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAg > 1:1526000/1‑76 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGcc > 1:593352/1‑76 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCg > 2:138365/1‑76 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCg > 2:1234273/1‑76 (MQ=255) cacnAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaaca > 1:1637922/1‑76 (MQ=255) acaAAAACGACAATATGAAAATTAATAGAGGAATAAAAGGC‑AATAAAAACGGCCCCCCGGAGGGAAGCCGcaacaa < 2:1572392/76‑1 (MQ=255) aaaaaCGGAAATACGAAAATNAATAGAGGACTGAAANGC‑AAAAAAAACGGACTCCCGGTGGGAAGCCGCAACAAAg < 2:1811881/76‑1 (MQ=255) aaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAg > 1:1625211/1‑76 (MQ=255) aaaaaCGACAATATAAAAATAAATAGAGGAATGAAAGGC‑AAAAAAACCGGCCTCCCGGAGGGAAGCCGCAACAAAg < 2:775628/76‑1 (MQ=255) aaaaCAACAATANGANAATTAATAGAGGAATGAANGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGt < 1:1497650/76‑1 (MQ=255) aaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTg > 1:667981/1‑76 (MQ=255) aaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTg > 1:1453284/1‑76 (MQ=255) aaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgc > 2:637393/1‑76 (MQ=255) aaCGACAATATAAAAATTAATAGAGGAATAAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgc < 1:1444150/76‑1 (MQ=255) aCGACAATATGAAAATTAATAGAGAAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgcg < 2:593283/76‑1 (MQ=255) aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGt > 2:585697/1‑76 (MQ=255) aaaTATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTg < 1:199666/75‑1 (MQ=255) tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt > 1:833575/1‑76 (MQ=255) tGAAAATTAATAGAGGANTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGCGCGCGTGGtttt > 1:232078/1‑76 (MQ=255) gaaaaaTTAAAACAGAAATGAAAGGC‑AAATAAAACGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGGGTGGtttt < 2:25994/74‑1 (MQ=255) gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 2:127801/1‑76 (MQ=255) aaaaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt > 2:378742/1‑76 (MQ=255) aaaTTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGTGTGGTTTTCTg < 1:1676213/76‑1 (MQ=255) aaNTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGCTTTTGNGc < 2:1492148/76‑1 (MQ=255) aTTAATAGAGAAATAAAAGG‑AAAAAAAACCGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa < 1:660961/76‑1 (MQ=255) ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCATGGTTTTATGCAg > 1:131254/1‑76 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 1:1329592/1‑76 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:1129320/1‑76 (MQ=255) aTAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGAGTGGTTTTATGCAGGATg > 1:803010/1‑76 (MQ=255) tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 1:821019/1‑76 (MQ=255) tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 1:128781/1‑76 (MQ=255) aaaaggaaaaaaaaggaaaaaaaACCGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 1:848322/73‑1 (MQ=255) gagaAATGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg < 2:1637921/76‑1 (MQ=255) aTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa > 1:868875/1‑76 (MQ=255) tGAAAGGC‑AAAAAAAACGGCTTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 2:156854/1‑76 (MQ=38) tGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa < 2:570883/76‑1 (MQ=255) gAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGCGGTTTTATGCAGGATGCGGCGTaaa > 1:132500/1‑76 (MQ=39) gAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGCGCGCGTGGTTTTATGCAGGATGCGGCGTaaa > 1:1156403/1‑76 (MQ=39) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:1679870/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:1424961/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 1:970672/1‑76 (MQ=255) | GATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAC > REL606/2131387‑2131528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |