Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	4,097,191	(CAACACT)1→2	coding (382/846 nt)	glpF ←	glycerol facilitator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 4097183	1 (0.020)	47 (0.930) +TCAACAC	36/120	0.2	98.0%	coding (390/846 nt)	glpF	glycerol facilitator
	?	REL606	4097184 =	1 (0.020)					coding (389/846 nt)	glpF	glycerol facilitator

TCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/4097110‑4097183 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCC  >  REL606/4097184‑4097256                                                                           |||||||                                                                          TCANCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATC                                                                                >  1:1635516/1‑76  CACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCA                                                                               <  1:790822/76‑1   ACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAA                                                                              <  2:1208019/76‑1   ACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAA                                                                              >  1:74036/1‑76      ATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACAC                                                                           >  1:607203/1‑76       TCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACT                                                                          <  1:934556/76‑1       TCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACT                                                                          <  1:1308526/76‑1         TCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCA                                                                        >  2:1088057/1‑76            ACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAACTAGAGAAAGTGCCAGCCAGATCAACACTCAACA                                                                     <  1:1679575/76‑1              TGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACT                                                                   <  1:1381296/76‑1              TGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACT                                                                   <  2:1271047/76‑1               GCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTT                                                                  >  2:1344459/1‑76                CGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGGGAAAGTGCCAGCCAGATCAACACTCATCACCTT                                                                 <  1:1681641/76‑1                CGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTT                                                                 <  1:810459/76‑1                 GAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTC                                                                <  2:333584/76‑1                 GAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTC                                                                >  2:99321/1‑76                  AAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACCCATTCA                                                               <  1:1734418/76‑1                  AAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCA                                                               >  1:640872/1‑76                  AAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCA                                                               >  2:698077/1‑76                  AAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCA                                                               <  2:926941/76‑1                   AAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAA                                                              <  1:1111415/76‑1                    AGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAAC                                                             >  2:1566188/1‑76                    AGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAAC                                                             >  2:721207/1‑76                        TGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTG                                                         <  1:965856/76‑1                               AATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAA                                                  >  1:789617/1‑76                                ATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAAC                                                 >  2:626828/1‑76                                     TATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCATCACTTTCAACGCTGCCGCGAACAATGT                                            <  1:1618504/76‑1                                     TATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGT                                            >  2:1505798/1‑76                                        GAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGAT                                         >  1:1225385/1‑76                                         AGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATG                                        >  2:1638997/1‑76                                         AGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCAAACAATGTGATG                                        <  2:76801/76‑1                                           GATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAG                                      >  1:519893/1‑76                                              TANGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGNCT                                   <  2:1485949/76‑1                                              TAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCT                                   <  1:695380/76‑1                                                 GGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGNGTCTGCT                                <  2:1471671/76‑1                                                 GGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCT                                >  1:1375915/1‑76                                                  GTAAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTC                               >  2:392173/1‑76                                                    AAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGA                             >  1:781668/1‑76                                                    AAGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGA                             >  1:522163/1‑76                                                     AGTAGAGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAA                            >  1:581190/1‑76                                                       TAGTGNAGGTGCCAGCCAGATCAACACTTAACACTTTCAACGCTGCCGCGAACAATGTGATNAGTCTGCTCGAAGT                          >  2:1833180/1‑76                                                        AGAGAAAGTGCCAGCCAGATCAACACTCAACACTCTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTC                         <  1:1505799/76‑1                                                          AGAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAGTGTGATGAGNCTGCTCGAAGTCGA                       <  1:8270/76‑1                                                           GAAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAA                      <  2:946672/76‑1                                                            AAAGTGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAA                     >  2:1334096/1‑76                                                                TGCCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATA                 >  2:1124626/1‑76                                                                  CCAGCCAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAA               >  1:465391/1‑76                                                                       CAGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTA          >  1:908995/1‑76                                                                        AGATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGGCTGCTCGGAGACGAAAAATAAATTGTAG         >  1:405185/1‑76                                                                          ATCAACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTA       >  2:1268345/1‑76                                                                           TCAACACTCAACACTTTAAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGACGAAAAATAAATTGTAGTAA      >  1:1820266/1‑76                                                                             AACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAG    <  2:1246445/76‑1                                                                              ACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGC   <  1:825875/76‑1                                                                              ACACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGC   >  1:987268/1‑76                                                                               CACTCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCC  <  1:721208/76‑1                                                                           |||||||                                                                          TCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/4097110‑4097183 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCC  >  REL606/4097184‑4097256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.