Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	3893609–3894994	3901930	6937–8322	18 [17]	[0] 60	[insJ‑5]–[yieO]	[insJ‑5],insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3652533	NA (NA)	55 (1.010)	43/146	0.2	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	3901931 =	0 (0.000)					coding (489/1428 nt)	yieO	predicted multidrug or homocysteine efflux system

GGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA  >  REL606/3893533‑3893626                                                                            |                   ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                    <  2:1042355/76‑1 (MQ=255)    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAGATGGAATAGCCCCTAATATGTCAAAGcc                 >  1:291511/1‑76 (MQ=255)      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa               >  2:779006/1‑76 (MQ=255)      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa               <  1:1316286/76‑1 (MQ=255)        aTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaaa             >  1:1384425/1‑76 (MQ=255)          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa           <  1:517088/76‑1 (MQ=255)          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa           <  1:1476641/76‑1 (MQ=255)          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa           >  2:1234168/1‑76 (MQ=255)          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa           >  2:1282495/1‑76 (MQ=255)            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc         >  2:1153001/1‑76 (MQ=255)             aCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAccc        >  2:1150593/1‑76 (MQ=255)              cGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCt       <  2:644928/76‑1 (MQ=40)               gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt      <  2:678068/76‑1 (MQ=39)                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt     >  1:680732/1‑76 (MQ=38)                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  >  1:1671093/1‑76 (MQ=32)                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  <  1:1524504/76‑1 (MQ=32)                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  >  1:898965/1‑76 (MQ=32)                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  >  2:1385922/1‑76 (MQ=32)                                                                            |                   GGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA  >  REL606/3893533‑3893626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑