| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,004,183 | C→T | intergenic (‑109/+493) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,004,183 | 0 | C | T | 98.7% | 295.0 / ‑4.2 | 77 | intergenic (‑109/+493) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (39/37); total (39/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
GCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCA > REL606/1004110‑1004255 | gCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTg < 1:576294/76‑1 (MQ=255) ccACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGc < 2:1747895/76‑1 (MQ=255) cACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCa > 2:986523/1‑76 (MQ=255) cACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCa < 1:1378273/76‑1 (MQ=255) cACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCa > 2:1649217/1‑76 (MQ=255) aCTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAg < 2:814056/76‑1 (MQ=255) cTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAgg > 1:730289/1‑76 (MQ=255) cTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAgg < 2:722388/76‑1 (MQ=255) cTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAgg > 2:1753317/1‑76 (MQ=255) cTGCCGTCAATAAGCTCCGCCAATAAAAATTTACGGAACTATTGATGAGAGCTTGATGTCTTTATGTGTTTGCAgg < 1:92969/76‑1 (MQ=255) gCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCa > 1:858678/1‑76 (MQ=255) gCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCa < 1:504733/76‑1 (MQ=255) gCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCa < 1:504736/76‑1 (MQ=255) ccGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCAt < 1:772895/76‑1 (MQ=255) gTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCt > 2:401784/1‑76 (MQ=255) gTCAATAAGTTCCGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGCCATCt > 2:68227/1‑76 (MQ=255) cAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCttt < 2:739487/76‑1 (MQ=255) cAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCttt > 2:1511224/1‑76 (MQ=255) cAAAAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCttt > 2:899320/1‑76 (MQ=255) aaTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTc < 2:858677/76‑1 (MQ=255) aTAAGTTCTGTTAATAAAAATTTACGGAACTATCGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTcc > 1:1679945/1‑76 (MQ=255) aTAAGTACTGTTAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTcc > 1:1679927/1‑76 (MQ=255) aaGTTCTGTCAATAAAAATTTACGGAACTATTGATGTGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCAt > 2:1463478/1‑76 (MQ=255) aaGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGCGTTTGCAGGCATCTTTCCAt > 2:1463457/1‑76 (MQ=255) aGTTCTGTCAATAAATATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCAtt < 1:1511225/76‑1 (MQ=255) aGTTCTGTCAATAAAAATTTCCGGCACTATTGATGAGAGTTTGGTGTCTTTATCTGTTAGCTGGCATCTTTCCAtt > 1:84518/1‑76 (MQ=255) aGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCAtt < 2:1312060/76‑1 (MQ=255) ttCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTaa < 1:816696/76‑1 (MQ=255) cTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTaaaa > 1:338770/1‑76 (MQ=255) tGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCGTTATGTGTTTGCAGGCATCTTTCCATTAAAAc < 1:956120/76‑1 (MQ=255) tGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCGTTATGTGTTTGCAGGCATCTTTCCATTAAAAc < 1:956121/76‑1 (MQ=255) cAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTaa > 2:85169/1‑76 (MQ=255) cAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTaa < 2:773146/76‑1 (MQ=255) aaTAAAAATTTACGGAACTATTGATGAGAGTTTTGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAAc < 2:682080/76‑1 (MQ=255) aaTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAAc > 1:423482/1‑76 (MQ=255) aaaaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGtt < 2:421115/76‑1 (MQ=255) aaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCg < 1:951256/76‑1 (MQ=255) tttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCt > 2:508124/1‑76 (MQ=255) tttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCt < 1:1313294/76‑1 (MQ=255) ttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTa > 1:1320960/1‑76 (MQ=255) aCGGAACTATTGATNAGAGTTTGGTGTCTTTATGTGTTTGCAGGCAGCTTTCCATTAAAACTAACGTTTCNCTAcc < 2:1835979/76‑1 (MQ=255) aCGGAACTATTGATGAGAGTTTGGTGTCTTCATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTAcc < 1:36762/76‑1 (MQ=255) cGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTNCATTAAAACTAACGTTTCGCTACCg > 2:124405/1‑76 (MQ=255) aCTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGaa < 2:549172/76‑1 (MQ=255) aCTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGaa < 1:244236/76‑1 (MQ=255) cTATTGATGAGAGTTTGGTGTNTTNATGTGTTTGCAGGCATCTTTCNATTAAAANTNACGTTTCGCTACCGTGaaa > 2:271848/1‑76 (MQ=255) cTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTNaaa < 1:1649922/76‑1 (MQ=255) cTATTGATGAGAGTTTGGCGTNTTNATGTGTTTGCAGGCATCTTTCNATTAAAANTNACGTTTCGCTACCGTGaaa > 2:271832/1‑76 (MQ=255) tATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAg > 1:503754/1‑76 (MQ=255) tATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAg > 1:503776/1‑76 (MQ=255) tgatgaGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGc > 2:631023/1‑76 (MQ=255) atgaGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTa < 2:1597969/76‑1 (MQ=255) atgaGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTa < 2:1035595/76‑1 (MQ=255) atgaGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTa < 2:423481/76‑1 (MQ=255) tgaGAGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTAc > 2:1749968/1‑76 (MQ=255) gagaGTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACa < 2:481404/76‑1 (MQ=255) agagTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaa > 2:204937/1‑76 (MQ=255) agagTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaa > 2:204962/1‑76 (MQ=255) agTTTGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaag > 2:1128077/1‑76 (MQ=255) gTTTGGTGTCTATATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaaga > 1:54863/1‑76 (MQ=255) tttGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCGNCaaagat < 2:1476942/76‑1 (MQ=255) ttGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagata < 2:536765/76‑1 (MQ=255) ttGGTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagata < 1:542606/76‑1 (MQ=255) tGGTGACTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataa > 1:122307/1‑76 (MQ=255) ggTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAGACTGACGTTTCGCTACCGAGAAAGTGCTACaaagataaa > 2:1458310/1‑76 (MQ=255) ggTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaa > 2:540871/1‑76 (MQ=255) ggTGTCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaa > 1:1422115/1‑76 (MQ=255) gtgtCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaag < 1:1184424/76‑1 (MQ=255) gtCTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaagat < 1:736468/76‑1 (MQ=255) cTTTATGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAttt < 2:751815/76‑1 (MQ=255) cTTTATGTGTTAGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAttt > 1:53808/1‑76 (MQ=255) aTGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGtt > 1:357679/1‑76 (MQ=255) aTGTGTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGtt < 2:1298626/76‑1 (MQ=255) tgtgTTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGttt > 1:697026/1‑76 (MQ=255) gtgtTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTc > 1:934992/1‑76 (MQ=255) tgtTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCa < 2:1099600/76‑1 (MQ=255) tgtTTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCa > 1:720028/1‑76 (MQ=255) | GCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCA > REL606/1004110‑1004255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |