Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	158.1 / NA	49	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/27);  total (22/27)

CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193707‑2193850                                                                         |                                                                        ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATccc                                                                      <  1:990085/76‑1 (MQ=255)   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                    >  1:831058/1‑76 (MQ=255)   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                    <  1:819164/76‑1 (MQ=255)    aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATGAGCCCCAATAACAGCAAAATGCCAATCCccac                                                                   <  2:1372964/76‑1 (MQ=255)    aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                   <  1:965189/76‑1 (MQ=255)    aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                   <  1:944233/76‑1 (MQ=255)      gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCCATCCccccca                                                                 >  1:1372390/1‑76 (MQ=255)       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCCCCAg                                                                >  1:450551/1‑76 (MQ=255)        aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCCATAACAGCAAAACCCCACTCCCCACCAGa                                                               >  1:1032131/1‑76 (MQ=255)        aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                               <  1:1414938/76‑1 (MQ=255)             gCGTCCAGGCGCGGGTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCCATCCCCGCCAGATAAAg                                                          <  1:157536/76‑1 (MQ=255)             gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                          <  2:682299/76‑1 (MQ=255)                 ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  2:1435202/76‑1 (MQ=255)                    ggCGCGGTTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg                                                   <  1:36507/76‑1 (MQ=255)                      cgcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCCCCAGATAAAGCATGACGcc                                                 >  2:1716454/1‑76 (MQ=255)                          gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                             <  1:358595/76‑1 (MQ=255)                          gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                             <  1:422131/76‑1 (MQ=255)                          gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                             <  1:1106143/76‑1 (MQ=255)                             ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          >  2:1764787/1‑76 (MQ=255)                             ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          >  2:1604599/1‑76 (MQ=255)                              gTCTGGTTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                         >  1:1233186/1‑76 (MQ=255)                              gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGGCGCCTGTATTaa                                         <  2:67717/76‑1 (MQ=255)                              gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                         >  1:1233166/1‑76 (MQ=255)                                cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                       >  1:175300/1‑76 (MQ=255)                                 tGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      >  2:885135/1‑76 (MQ=255)                                 tGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCAACCAGATAAAGCATGACGCCTGTATTAAACa                                      >  1:1239629/1‑76 (MQ=255)                                  ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     <  1:1327324/76‑1 (MQ=255)                                      gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 >  2:686107/1‑76 (MQ=255)                                      gTCAGAAATCAGCCCCTATGACAGNAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 >  1:159153/1‑76 (MQ=255)                                       tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                <  2:536246/76‑1 (MQ=255)                                       tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                <  2:654712/76‑1 (MQ=255)                                         ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                              <  2:627646/76‑1 (MQ=255)                                         ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                              <  2:48547/76‑1 (MQ=255)                                           aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                            <  2:1367245/76‑1 (MQ=255)                                              tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                         <  1:914070/76‑1 (MQ=255)                                                 gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                      <  2:402834/76‑1 (MQ=255)                                                     cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTGCCGATGGCGCa                  <  1:103406/76‑1 (MQ=255)                                                      aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                 >  1:796620/1‑76 (MQ=255)                                                      aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                 >  1:1324518/1‑76 (MQ=255)                                                       aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                <  2:960630/76‑1 (MQ=255)                                                         aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc              >  2:578072/1‑76 (MQ=255)                                                         aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc              >  1:514293/1‑76 (MQ=255)                                                           cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc            >  2:963541/1‑76 (MQ=255)                                                            aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa           <  1:457077/76‑1 (MQ=255)                                                              cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCGTCGGGCAca         >  2:636080/1‑75 (MQ=255)                                                              cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTATTTACCGATGGCGCATCGCGCAcc         >  1:1420569/1‑76 (MQ=255)                                                                aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg       >  2:1337264/1‑76 (MQ=255)                                                                aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg       >  2:1005356/1‑76 (MQ=255)                                                                 aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc      >  2:1173274/1‑76 (MQ=255)                                                                     ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  <  2:1718705/76‑1 (MQ=255)                                                                     ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  >  2:1368896/1‑76 (MQ=255)                                                                     ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  <  2:831057/76‑1 (MQ=255)                                                                         |                                                                        CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193707‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑