| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,169,030 | T→A | I1357N (ATC→AAC) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,169,030 | 0 | T | A | 97.6% | 153.4 / ‑3.5 | 41 | I1357N (ATC→AAC) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (27/13); total (28/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 | |||||||||||
ACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTG > REL606/4168957‑4169101 | aCCGAAGAAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAAcc > 1:655263/1‑76 (MQ=255) ccGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAAccc > 1:150428/1‑76 (MQ=255) gcagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAg < 2:5293/76‑1 (MQ=255) cagcCTGTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAgg < 2:1804626/76‑1 (MQ=255) cagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAgg > 1:1627925/1‑76 (MQ=255) cagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGCCTGAACCCGGCAgg > 1:663646/1‑76 (MQ=255) agcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAggg > 2:854447/1‑75 (MQ=255) ccGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTAc < 2:891651/76‑1 (MQ=255) ccGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTAc < 2:796769/76‑1 (MQ=255) gTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCg < 1:696464/76‑1 (MQ=255) gCGGGCAAACTCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGtt < 2:1268635/76‑1 (MQ=255) cGGGCAAGCGCGGCGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTa < 2:79967/76‑1 (MQ=255) cGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTa > 2:579283/1‑76 (MQ=255) cGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTa < 2:1132770/76‑1 (MQ=255) cGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGCACCGGTTa < 2:25560/76‑1 (MQ=255) cAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTAcgcg > 1:1476614/1‑76 (MQ=255) aaaCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGt < 2:791816/76‑1 (MQ=255) aaaCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGt > 1:673448/1‑76 (MQ=255) gcgACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTacca < 1:818312/76‑1 (MQ=255) nacgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTaccacc > 1:117491/2‑76 (MQ=255) acgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTAccacca < 1:485403/76‑1 (MQ=255) gaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAgg > 1:201725/1‑76 (MQ=255) aaCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGAACCGGTTACGCGAAACAACAGGa > 2:676866/1‑76 (MQ=255) aCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGAt > 2:1152842/1‑76 (MQ=255) ccTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGt > 2:1038227/1‑76 (MQ=255) cTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTc > 1:1623609/1‑76 (MQ=255) cTGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTc > 2:360566/1‑76 (MQ=255) tGAAAGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCg > 1:1319496/1‑76 (MQ=255) aaaGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGcc > 1:737840/1‑76 (MQ=255) aaaGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGcc > 1:899451/1‑76 (MQ=255) aaaGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGcc > 2:156113/1‑76 (MQ=255) aaGAGAACGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCg > 2:205779/1‑76 (MQ=255) aaCGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGtgctg > 2:465562/1‑76 (MQ=255) aaCGTTATCGGGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGtgctg < 1:645072/76‑1 (MQ=255) aCGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGtgctgc > 2:1038900/1‑76 (MQ=255) cGTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCg < 2:1181508/76‑1 (MQ=255) gTTATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCgg > 1:1191774/1‑76 (MQ=255) tATCGTGGGTCGTCTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCgggg > 1:134467/1‑75 (MQ=255) tATCGTGGGGCGTCTGCACCCGGCAGGTACCGGTTACGCGCACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGt < 2:1620066/76‑1 (MQ=255) cGTGGGTCGTCTGCACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCGGCGGGGGaa > 1:1214060/1‑76 (MQ=255) cgtcTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCgg > 1:1685767/1‑76 (MQ=255) cgtcTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCgg > 1:1650868/1‑76 (MQ=255) cgtcTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGGGCTGCGGGTGAAGCGCCgg > 1:111061/1‑76 (MQ=255) tcTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGGGAAGCTCCGGCt > 1:716238/1‑76 (MQ=255) tcTGAACCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGGGAAGCTCCGGCt > 1:278743/1‑76 (MQ=255) cTGATCCCGGCCGGAACCGGGTACGCGTACCACCAGGATCGAATGCGTCGCCGTGCTGCGGGGGAAGCTCCGGcgg > 2:623131/1‑74 (MQ=255) | ACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTG > REL606/4168957‑4169101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |