| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,219,546 | T→G | I97S (ATT→AGT) | yjbA → | phosphate‑starvation‑inducible protein PsiE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,219,546 | 0 | T | G | 94.8% | 225.9 / 1.1 | 58 | I97S (ATT→AGT) | yjbA | phosphate‑starvation‑inducible protein PsiE |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base G (35/20); total (37/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CTGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCATTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTACT > REL606/4219473‑4219618 | cTGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGtg < 1:297542/76‑1 (MQ=255) tGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCATtgt > 2:1759502/1‑76 (MQ=255) tGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCATtgt > 1:1719820/1‑76 (MQ=255) gATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGtgtg > 2:1148875/1‑76 (MQ=255) gATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGtgtg < 1:1630059/76‑1 (MQ=255) ttGTGAATTTCTTTCAGTCCGTTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTgcg < 1:400820/76‑1 (MQ=255) tgtgAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTgcgc > 2:164812/1‑76 (MQ=255) tgAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCtt > 1:1052482/1‑76 (MQ=255) tgAAGTACTTTCAGTCCGGTTTTCACGTCCCGCTCCGTTGCTTTGTCTATTTTGGGATCACCGCCAGTGTGCGCtt < 2:668019/76‑1 (MQ=255) aaGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGa > 2:1535593/1‑76 (MQ=255) aGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGat < 1:588428/76‑1 (MQ=255) tACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGatca > 2:309689/1‑76 (MQ=255) cTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGatcatc > 2:397663/1‑76 (MQ=255) atttCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGatcatc > 1:959283/2‑76 (MQ=255) ttCAGTCCTGTTTTCCCTTCCCGCTCGGTTACTTTGTCTATATTGGGATCGCCGCCATTGTGCGCTTGATCAtcgt < 1:131686/76‑1 (MQ=255) ttCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATGGGGATCACCGCCAGTGTGCGCTTGATCAtcgt < 2:1315814/76‑1 (MQ=255) cAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCAtcgtcg > 1:1405485/1‑76 (MQ=255) cAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCAtcgtcg > 1:258294/1‑76 (MQ=255) aGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGa > 1:492209/1‑76 (MQ=255) tCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATc > 2:1229621/1‑76 (MQ=255) ggTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATa < 2:1263172/76‑1 (MQ=255) gTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATaa < 2:666408/76‑1 (MQ=255) gTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATaa < 2:1577770/76‑1 (MQ=255) ttttCACTTCCCTCTACGTTACTTTGTCTATATTGGGATCTCCGCCAGTGTGCGCTTGATCATCGTCGATCATaaa < 2:1628783/76‑1 (MQ=255) ttttCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATaaa > 2:367041/1‑76 (MQ=255) tttCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAAt < 2:1704398/76‑1 (MQ=255) tttCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAAt < 1:1633155/76‑1 (MQ=255) tCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGGCGATCATAAATCg > 1:919170/1‑76 (MQ=255) cTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCa > 2:1170819/1‑76 (MQ=255) ttCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCAc > 2:266907/1‑76 (MQ=255) tCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACt > 2:1764932/1‑76 (MQ=255) cccccCTACGTTACTTTGTCTATATTGGAATCCCCGCCAGTGTGCGCTGGATCTTCGTCGGTCATAAATCGCCACt < 2:914061/75‑1 (MQ=255) ccGCTGCGTTTCTTTGTATATATGGGGATCCCCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTgg < 1:828611/76‑1 (MQ=255) cGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGa > 1:58101/1‑76 (MQ=255) cGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGa > 2:1334396/1‑76 (MQ=255) ctgcGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtg > 1:1340384/4‑76 (MQ=255) cTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtg < 2:1088046/76‑1 (MQ=255) tACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgt < 1:1444825/76‑1 (MQ=255) aCGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgtg > 1:768545/1‑76 (MQ=255) aCGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgtg > 1:1328372/1‑76 (MQ=255) aCGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgtg > 1:1294326/1‑76 (MQ=255) aCGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgtg > 1:969634/1‑76 (MQ=255) cGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATgtgt > 2:1171323/1‑76 (MQ=255) ttACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTg > 2:1507137/1‑76 (MQ=255) tACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGa > 1:1657011/1‑76 (MQ=255) tACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGa > 1:373638/1‑76 (MQ=255) aCTTTGTTTATGTTGGAATCGCCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGAt < 1:480342/76‑1 (MQ=255) aCTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGAt > 1:566332/1‑76 (MQ=255) aCTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGAt < 2:211186/76‑1 (MQ=255) ttGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTa < 1:1375466/76‑1 (MQ=255) cTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCg > 2:1566703/1‑76 (MQ=255) cTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCg > 1:783860/1‑76 (MQ=255) tataTTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCgg < 1:701519/76‑1 (MQ=255) tataTTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCgg > 1:28953/1‑76 (MQ=255) atatTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGc > 1:713242/1‑76 (MQ=255) atatTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGc > 2:248926/1‑76 (MQ=255) tatTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCt > 2:1304530/1‑76 (MQ=255) tatTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCt > 1:1412930/1‑76 (MQ=255) tGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCg > 1:884076/1‑76 (MQ=255) aTCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATcc > 2:553730/1‑76 (MQ=255) tCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATCCt > 2:1099761/1‑76 (MQ=255) ccGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCCACTCGGCTGCGATCCTGtt < 2:1469719/76‑1 (MQ=255) gCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTAc < 1:1002577/76‑1 (MQ=255) ccAGTGTGCGCTGGAGCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCGACTCGGCTGCGATCCTGTTACt < 2:319895/76‑1 (MQ=255) | CTGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCATTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTACT > REL606/4219473‑4219618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |