| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,411,844 | G→T | F130L (TTC→TTA) | ECB_04076 ← | putative oxoacyl reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,411,844 | 0 | G | T | 93.5% | 150.6 / 1.8 | 47 | F130L (TTC→TTA) | ECB_04076 | putative oxoacyl reductase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (19/24); total (20/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01 | |||||||||||
CTGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTTCGAG > REL606/4411776‑4411915 | cTGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCCGTAAGCGCTTACTTAATGCCt > 1:1532438/1‑76 (MQ=255) tGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTg > 2:288375/1‑76 (MQ=255) tGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCCGCCGTGCCAGCAAACGCCTACTTAATGcccg > 1:822321/1‑74 (MQ=255) tGACGAAAATAATCGAGCGGTGCGGCAGCGCCGCTTCCGGGTTTGGCTGGGGCAGATCGCGTTTACTTCATGccgg > 2:229277/1‑74 (MQ=255) aCGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAg < 2:409858/76‑1 (MQ=255) aaaaTAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGGGCCAGTAAGCGCTTACTTAATGCCTGAGCaa > 2:1383304/1‑76 (MQ=255) aaaTAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAg < 1:716437/76‑1 (MQ=255) aaaTAAGCGAGCGGTGCGGCAGGTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAg < 1:700858/76‑1 (MQ=255) ataatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGga > 2:782509/1‑76 (MQ=255) ataatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGga < 2:147022/76‑1 (MQ=255) taatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaa < 2:1607825/76‑1 (MQ=255) aatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaag < 1:1745043/76‑1 (MQ=255) aatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaag < 2:963071/76‑1 (MQ=255) aatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaag < 1:744417/76‑1 (MQ=255) aatCGAGAGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaag > 1:1170987/1‑76 (MQ=255) atCGAGCGGTGCGGCAGCTCCGCTTCCGGGTTCGGCTGTGCCAGTAAGCGATTACTTAATGCCTGAGCAAGgaaga > 2:155790/1‑76 (MQ=255) tCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaagaa < 1:1755968/76‑1 (MQ=255) tCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaagaa < 1:1184245/76‑1 (MQ=255) cGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAAc > 2:1092174/1‑76 (MQ=255) cGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAAc < 1:273386/76‑1 (MQ=255) gAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGGCAGGAAGCGCCTACTTAATGCCTGAGGAAGggagaaag > 2:386010/1‑74 (MQ=255) gCGGTGCGGCAGCTCCGCTTCCGGTGTCGGCTGTGCTAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGgc < 2:99633/76‑1 (MQ=255) cGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAATAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGgcg > 2:52825/1‑76 (MQ=255) ggTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTTCTTAATGCCTGAGCCAGGAAGAACGgcgc > 2:722411/1‑76 (MQ=255) ggTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGgcgc < 1:506873/76‑1 (MQ=255) gTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGgcgcg > 1:197515/1‑76 (MQ=255) cGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGATGAACGGCGCGCgg < 1:1201612/76‑1 (MQ=255) ggCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCggg > 1:749012/1‑76 (MQ=255) gCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGgt < 2:1388768/76‑1 (MQ=255) gCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGgt < 2:669982/76‑1 (MQ=255) aGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAACGGCGCGCGGgtgt > 1:126987/1‑76 (MQ=255) cTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTa < 2:1051733/76‑1 (MQ=255) cGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATg < 2:1744272/76‑1 (MQ=255) cGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATg > 2:577409/1‑76 (MQ=255) cTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGc < 2:755966/76‑1 (MQ=255) tCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCaa < 1:585402/76‑1 (MQ=255) tttCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGttt > 1:480124/1‑76 (MQ=255) tttCGGCTGTGCCACTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGttt > 1:983756/1‑76 (MQ=255) ttCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGtttt > 1:425421/1‑76 (MQ=255) tCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTg < 2:1626076/76‑1 (MQ=255) cGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGa < 1:940055/76‑1 (MQ=255) gCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATc < 1:408576/76‑1 (MQ=255) gCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATc < 1:1424458/76‑1 (MQ=255) cTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCg > 1:467288/1‑76 (MQ=255) tgtgCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATTTTTTGATCGa < 2:180660/76‑1 (MQ=255) gtgCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGaa > 1:789600/1‑76 (MQ=255) aaGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTg > 2:219946/1‑76 (MQ=255) cgcTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGtt < 2:1065530/76‑1 (MQ=255) cgcTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGtt > 1:87350/1‑76 (MQ=255) cgcTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGtt > 1:1083217/1‑76 (MQ=255) gcTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTtc > 2:2146/1‑76 (MQ=255) cttacttaATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTtcg < 1:309573/76‑1 (MQ=255) ttACTCAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTtcga < 2:1812099/76‑1 (MQ=255) tACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTtcgag > 1:905370/1‑76 (MQ=255) | CTGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTTCGAG > REL606/4411776‑4411915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |