Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	148.0 / NA	46	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/22);  total (24/22)

CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGC  >  REL606/2193707‑2193849                                                                          |                                                                       ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATccc                                                                     >  2:1335669/1‑76 (MQ=255) ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAAAGCCAATccc                                                                     >  1:1479538/1‑76 (MQ=255) ccGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATccc                                                                     >  2:469197/1‑76 (MQ=255)       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                               >  2:683729/1‑76 (MQ=255)       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                               <  1:190329/76‑1 (MQ=255)          ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTA‑GGAAATCCGCCCCCATAATAGAAAAACGCCAAACCCCACCCGATa                                                            >  2:1109436/1‑76 (MQ=255)            ggCGTCCAACCGCGGCAGGACTGGCTGCA‑CGCGACCAGCCCCAACATCAGCAAAACACCAAGTCCCACCAGATaaa                                                          >  2:268441/1‑76 (MQ=255)             gCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                         <  2:290969/76‑1 (MQ=255)                 ccAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                     <  1:1562091/76‑1 (MQ=255)                  cAGGCGCGGCTGGTCTTGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                    >  2:246313/1‑76 (MQ=255)                  cAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                    <  2:1699995/76‑1 (MQ=255)                  cAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                    >  2:1000542/1‑76 (MQ=255)                   aGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                   <  1:835169/76‑1 (MQ=255)                     gcgcGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGc                                                 >  1:618700/1‑76 (MQ=255)                       gcgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                               <  1:1308552/76‑1 (MQ=255)                        cgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                              >  1:1433018/1‑76 (MQ=255)                        cgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                              >  1:591220/1‑76 (MQ=255)                          gCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                            <  2:1600596/76‑1 (MQ=255)                           cTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                           <  2:232357/76‑1 (MQ=255)                            tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                          >  2:979893/1‑76 (MQ=255)                            tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                          <  1:1207009/76‑1 (MQ=255)                             ggTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                         <  1:198446/76‑1 (MQ=255)                             ggTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                         >  1:219673/1‑76 (MQ=255)                               tCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                       <  2:403747/76‑1 (MQ=255)                                cTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                      >  1:1221266/1‑76 (MQ=255)                                cTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                      >  2:1287755/1‑76 (MQ=255)                                 tGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                     >  1:1781968/1‑76 (MQ=255)                                  ggCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                    >  1:1453189/1‑76 (MQ=255)                                    cTGTCGGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                   >  1:387955/1‑76 (MQ=255)                                    cTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                  >  2:1552440/1‑76 (MQ=255)                                     tGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                 >  1:944650/1‑76 (MQ=255)                                       tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCGCCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                               <  2:931408/76‑1 (MQ=255)                                        c‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                              <  2:887815/76‑1 (MQ=255)                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                             <  2:448678/76‑1 (MQ=255)                                           gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                            <  2:245088/76‑1 (MQ=255)                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                           >  2:1085126/1‑76 (MQ=255)                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                           >  1:1607872/1‑76 (MQ=255)                                              aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAATCATGACGCCTGTATTAAACAGGCTTTTTACCg                         <  2:116736/76‑1 (MQ=255)                                                  gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                     <  1:1041807/76‑1 (MQ=255)                                                    cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                   >  1:340358/1‑76 (MQ=255)                                                    cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                   <  1:449409/76‑1 (MQ=255)                                                     ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGGTTTTTACAGATGgcgc                  >  1:199127/1‑76 (MQ=255)                                                          aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc             >  1:899682/1‑76 (MQ=255)                                                             aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa          <  2:4358/76‑1 (MQ=255)                                                             aGCAAAACACCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa          <  2:1196065/76‑1 (MQ=255)                                                               cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAACCAGGCTTTTTACCGCTGGCGCATCGCGCAca        >  1:72968/1‑75 (MQ=255)                                                               cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc        <  2:1722503/76‑1 (MQ=255)                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg      <  2:970619/76‑1 (MQ=255)                                                                   aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGTATCGCGCACCAgcc    <  2:235504/76‑1 (MQ=255)                                                                     gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc  >  2:1814981/1‑76 (MQ=255)                                                                          |                                                                       CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGC  >  REL606/2193707‑2193849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑