| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,328,951 | A→G | S10P (TCC→CCC) | mreB ← | cell wall structural complex MreBCD, actin‑like component MreB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,328,951 | 0 | A | G | 95.2% | 232.5 / 1.8 | 62 | S10P (TCC→CCC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (33/26); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
TTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCG > REL606/3328878‑3329021 | ttCAATACGATGCCTTGTCCTTTTACATAAATGAGTGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGgaa > 1:1329226/1‑76 (MQ=255)ttCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGgaa < 2:424100/76‑1 (MQ=255) aTACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACAt > 2:1050395/1‑76 (MQ=255) aTACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACAt < 1:1813140/76‑1 (MQ=255) tACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTTATTGGGAAACagg > 2:836558/1‑74 (MQ=255) tACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATg < 2:501404/76‑1 (MQ=255) aCGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGc < 1:1503930/76‑1 (MQ=255) aTGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCAc < 1:974442/76‑1 (MQ=255) aTGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCAc > 1:423901/1‑76 (MQ=255) gCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGa > 2:1779596/1‑76 (MQ=255) cTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGaaa > 1:1058261/1‑76 (MQ=255) tGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAtt > 2:980977/1‑76 (MQ=255) tGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAtt > 1:767100/1‑76 (MQ=255) gTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAttt > 1:581802/1‑76 (MQ=255) gTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAttt > 1:517079/1‑76 (MQ=255) tCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAAtttt < 1:97889/76‑1 (MQ=255) ttttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTc < 1:1042830/76‑1 (MQ=255) tttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCa < 2:1545278/76‑1 (MQ=255) tttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCa < 1:1602444/76‑1 (MQ=255) tttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCa < 2:1580660/76‑1 (MQ=255) tttACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCa < 1:634570/76‑1 (MQ=255) tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc > 2:845035/1‑76 (MQ=255) tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc < 1:1356793/76‑1 (MQ=255) tACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAAc < 2:672119/76‑1 (MQ=255) cATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACAt > 1:371214/1‑76 (MQ=255) aTAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATa < 2:940848/76‑1 (MQ=255) aaaTGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACt > 1:1729462/1‑76 (MQ=255) tGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGCAATTTTTTCAACATACTAAg > 2:1066527/1‑76 (MQ=255) gAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAgg < 1:265464/76‑1 (MQ=255) gAGGGTATTCGCAGTACCCAGGTC‑ATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg > 2:1441551/1‑76 (MQ=255) aGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg < 1:718404/76‑1 (MQ=255) aGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg > 1:948300/1‑76 (MQ=255) aGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg > 1:1563078/1‑76 (MQ=255) aGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAggg > 2:1183565/1‑76 (MQ=255) ggTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGAt < 2:51300/76‑1 (MQ=255) gTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATa > 1:651746/1‑76 (MQ=255) aTTCGCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAAt > 1:906320/1‑76 (MQ=255) gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg < 1:1241772/76‑1 (MQ=255) gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg > 1:1288108/1‑76 (MQ=255) gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg > 2:713969/1‑76 (MQ=255) gCAGTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg < 1:297959/76‑1 (MQ=255) gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa < 1:1353139/76‑1 (MQ=255) gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa > 1:553434/1‑76 (MQ=255) gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa < 1:1368475/76‑1 (MQ=255) gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa > 2:188349/1‑76 (MQ=255) gTACCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa > 1:1259374/1‑76 (MQ=255) aCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGc > 1:110329/1‑76 (MQ=255) aCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGc > 1:1378535/1‑76 (MQ=255) aCCCAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGc > 1:827987/1‑76 (MQ=255) ccAGGTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTg < 2:51884/76‑1 (MQ=255) ggTCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTgggg < 2:1668093/76‑1 (MQ=255) tCAATGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCg > 2:687323/1‑76 (MQ=255) aaTGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGga > 1:410308/1‑76 (MQ=255) tGGACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaa < 2:3428/76‑1 (MQ=255) tgACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCTgaaca > 2:1829690/2‑74 (MQ=255) tgACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaa > 2:1348611/2‑76 (MQ=255) ggACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACACACTAAGGGATAATCCTGTAATCTGGGGCGgaaaa > 2:527321/1‑76 (MQ=255) gACAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaag < 2:1001903/76‑1 (MQ=255) aaaaGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaaga < 1:929804/74‑1 (MQ=255) aCAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaaga < 1:805810/76‑1 (MQ=255) aCAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaaga > 2:624692/1‑76 (MQ=255) cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa < 2:1666901/76‑1 (MQ=255) cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa < 1:327321/76‑1 (MQ=255) cAAGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa < 1:1015719/76‑1 (MQ=255) aaGTCATTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaaa > 2:102659/1‑76 (MQ=255) aTTGGGAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCg > 2:1428266/1‑76 (MQ=255) | TTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCG > REL606/3328878‑3329021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |