| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,438,531 | T→G | D110A (GAC→GCC) | fbp ← | fructose‑1,6‑bisphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,438,531 | 0 | T | G | 96.9% | 390.2 / ‑2.2 | 97 | D110A (GAC→GCC) | fbp | fructose‑1,6‑bisphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base G (39/55); total (40/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCA > REL606/4438458‑4438602 | aaCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc < 1:150724/76‑1 (MQ=255)aaCGCGGCGGCAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc < 2:1374842/76‑1 (MQ=255) cgcgGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCAt < 2:403715/76‑1 (MQ=255) cggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCa < 2:1863088/76‑1 (MQ=255) ggcggTAGATGGAGAAAATGGTACCGACAGAGGCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAg < 1:255615/76‑1 (MQ=255) ggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAg > 1:1116344/1‑76 (MQ=255) ggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAg > 1:514650/1‑76 (MQ=255) ggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAg < 2:434777/76‑1 (MQ=255) cggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcg < 1:244545/76‑2 (MQ=255) cggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGca > 1:275581/1‑76 (MQ=255) cggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGca < 2:669767/76‑1 (MQ=255) gTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacc < 2:1629183/76‑1 (MQ=255) gTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacc < 1:1558315/76‑1 (MQ=255) tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacca > 1:1653359/1‑76 (MQ=255) aGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcaccac < 1:1268662/76‑1 (MQ=255) gATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACg > 2:95378/1‑76 (MQ=255) aTGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGt > 2:1107793/1‑76 (MQ=255) ggAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAt > 2:1671344/1‑76 (MQ=255) gaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAtttt < 2:593038/76‑1 (MQ=255) aaaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTg > 2:279666/1‑76 (MQ=255) aaaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTg < 2:1978759/76‑1 (MQ=255) aaaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTg < 2:1177261/76‑1 (MQ=255) aaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGc < 2:222472/76‑1 (MQ=255) aaaTGGTACCGACAGAGACGTTAACATCGAGGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGc < 2:142606/76‑1 (MQ=255) agggTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgt < 2:1731238/74‑1 (MQ=255) tGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgtg > 1:1659670/1‑76 (MQ=255) tGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgtg < 2:452386/76‑1 (MQ=255) gTACGGACAGAGACTGTAACATCGATGGTGGTCGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGtt < 2:329068/76‑1 (MQ=255) tACCGACAGAGACGTTAACATCGATGTTGGACGAGTCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTc > 2:1800431/1‑76 (MQ=255) tACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTc > 2:1086926/1‑76 (MQ=255) tACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTc > 2:949354/1‑76 (MQ=255) aCCGACATGGACGTTAACACCGGGGTGGAAAGAGACATCCAGGGGGGCCATCAGGACCACGTATTTTGCGTGTTca < 2:761789/76‑1 (MQ=255) aCCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca > 2:712076/1‑76 (MQ=255) aCCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca > 1:825582/1‑76 (MQ=255) ccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcac < 2:441090/76‑1 (MQ=255) ccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcac < 2:964109/76‑1 (MQ=255) cGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca < 1:806508/76‑1 (MQ=255) gACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAg < 1:632788/76‑1 (MQ=255) aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc < 1:1629222/76‑1 (MQ=255) aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc > 2:74115/1‑76 (MQ=255) gagaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCtt < 2:1290026/76‑1 (MQ=255) agaAGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTc < 2:606104/76‑1 (MQ=255) gaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCa < 1:682363/76‑1 (MQ=255) aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 2:1156750/76‑1 (MQ=255) aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 1:592827/76‑1 (MQ=255) cGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa < 1:905924/76‑1 (MQ=255) cGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa > 1:418530/1‑76 (MQ=255) cGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa > 1:131935/1‑76 (MQ=255) gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg < 2:224244/76‑1 (MQ=255) gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg < 2:1870605/76‑1 (MQ=255) gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg < 1:711026/76‑1 (MQ=255) ttAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga < 2:1343457/76‑1 (MQ=255) ttAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga > 2:1762236/1‑76 (MQ=255) tgaaCGTCGATGTTGGCCGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga < 2:348068/74‑1 (MQ=255) tAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac > 2:1083837/1‑76 (MQ=255) tAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac < 1:612725/76‑1 (MQ=255) tAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac > 1:1360895/1‑76 (MQ=255) tAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac < 1:799408/76‑1 (MQ=255) aaCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg > 2:1977239/1‑76 (MQ=255) aaCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg > 1:17469/1‑76 (MQ=255) aCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCACAgacga > 2:780729/1‑76 (MQ=255) aCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacga < 2:660691/76‑1 (MQ=255) cATCGATGTGGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAGAgacgac < 1:1277923/76‑1 (MQ=255) aTCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa < 2:1351692/76‑1 (MQ=255) gATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtc > 2:553763/1‑76 (MQ=255) gATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtc < 1:1663333/76‑1 (MQ=255) aTGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtct > 2:902651/1‑76 (MQ=255) gTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACCGCCTCCCAAGACGACAATCTCa > 2:520190/1‑76 (MQ=255) ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt > 1:1055651/1‑76 (MQ=255) ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt > 1:1366773/1‑76 (MQ=255) tGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtc > 1:1306225/1‑76 (MQ=255) tGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtc > 1:135897/1‑76 (MQ=255) gggcGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtct < 2:1055651/73‑1 (MQ=255) gACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtctt < 1:73389/76‑1 (MQ=255) aCGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttc < 2:1175315/76‑1 (MQ=255) cGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttct < 1:1012836/76‑1 (MQ=255) cGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttct > 2:1113849/1‑76 (MQ=255) gAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt < 1:1740963/76‑1 (MQ=255) gCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttcttct > 1:1236259/1‑76 (MQ=255) ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 2:321119/76‑1 (MQ=255) ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 1:149936/76‑1 (MQ=255) cATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCttcttcttc < 1:56119/76‑1 (MQ=255) cATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCttcttcttc < 1:1991394/76‑1 (MQ=255) cATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCttcttcttc > 1:854419/1‑76 (MQ=255) cATCCAGAGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCttcttcttc > 1:1275096/1‑76 (MQ=255) aTCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCa < 2:1891977/76‑1 (MQ=255) ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga > 1:1857915/1‑76 (MQ=255) ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga < 2:1439182/76‑1 (MQ=255) ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga > 2:672031/1‑76 (MQ=255) ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga > 2:681031/1‑76 (MQ=255) cAGGGGGGCCATCAGCGCCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag < 2:846561/76‑1 (MQ=255) cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCatag > 1:1626055/1‑76 (MQ=255) cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag < 2:626014/76‑1 (MQ=255) cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag > 2:1242590/1‑76 (MQ=255) cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag > 2:1506894/1‑76 (MQ=255) ggggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGc < 2:1599578/76‑1 (MQ=255) ggggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGc < 1:1800431/76‑1 (MQ=255) ggggTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:593342/76‑1 (MQ=255) ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:412593/76‑1 (MQ=255) ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:327113/76‑1 (MQ=255) | AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCA > REL606/4438458‑4438602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |