| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,004,196 | T→A | intergenic (‑122/+480) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,004,196 | 0 | T | A | 88.0% | 264.8 / 23.5 | 75 | intergenic (‑122/+480) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base A (25/41); total (28/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
ATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATA > REL606/1004123‑1004267 | aTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTAcc > 2:1016906/1‑76 (MQ=255) aaGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCAt < 2:757651/76‑1 (MQ=255) gTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTa < 2:720661/76‑1 (MQ=255) gTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTa < 2:1136816/76‑1 (MQ=255) ttCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTaa < 2:1000975/76‑1 (MQ=255) tCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTaaa > 1:1894969/1‑76 (MQ=255) cAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTaa > 2:216094/1‑76 (MQ=255) aTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACg < 1:185130/76‑1 (MQ=255) tttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCt < 1:981348/76‑1 (MQ=255) cGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCg < 2:1409049/76‑1 (MQ=255) cGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCg < 1:1806652/76‑1 (MQ=255) cGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCg > 2:191334/1‑76 (MQ=255) cGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCg > 2:1151486/1‑76 (MQ=255) cGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCg > 2:316616/1‑76 (MQ=255) aaCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGa < 1:642078/76‑1 (MQ=255) tATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAg < 1:30967/76‑1 (MQ=255) aTTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGt < 2:1217250/76‑1 (MQ=255) ttGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTg < 1:696113/76‑1 (MQ=255) ttGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTg > 2:1238723/1‑76 (MQ=255) atgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTa > 2:622476/1‑76 (MQ=255) gagaGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACa < 1:31455/76‑1 (MQ=255) agagTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaa < 1:1675861/76‑1 (MQ=255) gagTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaa > 1:263552/1‑76 (MQ=255) agTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaag < 1:782908/76‑1 (MQ=255) gTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaaga > 2:1912655/1‑76 (MQ=255) gTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaaga < 2:1329597/76‑1 (MQ=255) gTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaaga > 1:1840967/1‑76 (MQ=255) gTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaaga < 1:1819666/76‑1 (MQ=255) tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat > 1:481211/1‑76 (MQ=255) tttGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat < 1:926051/76‑1 (MQ=255) ttGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagata > 1:582344/1‑76 (MQ=255) ttGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagata < 1:954573/76‑1 (MQ=255) tGGTGTCTTTATGTATCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataa > 1:1070255/1‑76 (MQ=255) ggTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaa < 1:1279682/76‑1 (MQ=255) ggTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaa < 2:1312206/76‑1 (MQ=255) gtgtCTTTATGTGTGTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaag < 1:991900/76‑1 (MQ=255) gtgtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaag < 2:297244/76‑1 (MQ=255) gtgtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaag < 2:683771/76‑1 (MQ=255) gtgtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaag < 1:171828/76‑1 (MQ=255) gtgtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaag < 2:809480/76‑1 (MQ=255) gtgtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaag < 2:1701928/76‑1 (MQ=255) tgtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaaga > 2:1994153/1‑76 (MQ=255) gtCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaagat < 1:1006658/76‑1 (MQ=255) gtCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaagat < 1:1980480/76‑1 (MQ=255) tCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAtt < 1:44069/76‑1 (MQ=255) tCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAtt > 1:349463/1‑76 (MQ=255) tCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAtt < 2:1586679/76‑1 (MQ=255) tCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAtt < 2:610790/76‑1 (MQ=255) tCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAtt > 1:1206833/1‑76 (MQ=255) cTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAttt > 1:1451318/1‑76 (MQ=255) tttATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAttta < 2:1433395/76‑2 (MQ=255) tttATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTg < 2:875446/76‑1 (MQ=255) tATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGt > 2:1039081/1‑76 (MQ=255) aTGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGtt < 2:1407632/76‑1 (MQ=255) aTGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGtt < 2:28857/76‑1 (MQ=255) tgtCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCa < 2:682174/76‑1 (MQ=255) gtCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa < 1:659562/76‑1 (MQ=255) gtCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa > 1:348641/1‑76 (MQ=255) tCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaa > 2:1980219/1‑76 (MQ=255) tCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaa < 1:297674/76‑1 (MQ=255) tCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaa > 2:56972/1‑76 (MQ=255) cTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa < 1:1554473/76‑1 (MQ=255) cTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa < 1:466118/76‑1 (MQ=255) tGCAGGCATCTTACTATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaaa > 1:1073352/1‑76 (MQ=255) gCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAg > 2:1270230/1‑76 (MQ=255) gCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAg < 1:638389/76‑1 (MQ=255) cAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGa < 1:1599842/76‑1 (MQ=255) aGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaa < 2:792067/76‑1 (MQ=255) aGGCATCTTACCACTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaa > 1:1309299/1‑76 (MQ=255) ggCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaa < 1:1034878/76‑1 (MQ=255) gCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaa > 1:1017810/1‑76 (MQ=255) gCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGATACAAAGATAAAGATTTGGTTTCAAAAAGaaaa > 1:1017781/1‑76 (MQ=255) cATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaa < 1:20059/76‑1 (MQ=255) cATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaa > 2:1228488/1‑76 (MQ=255) tCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAata < 2:1175139/76‑1 (MQ=255) | ATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATA > REL606/1004123‑1004267 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |