| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,290,813 | C→T | Q260* (CAG→TAG) | hnr → | response regulator of RpoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,290,813 | 0 | C | T | 98.3% | 226.4 / ‑3.4 | 60 | Q260* (CAG→TAG) | hnr | response regulator of RpoS |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (31/28); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01 | |||||||||||
CAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATG > REL606/1290741‑1290885 | cAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGt > 2:1863301/1‑76 (MQ=255)cAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGt < 2:547493/76‑1 (MQ=255)cAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGt < 2:1570367/76‑1 (MQ=255) aaCGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGtt < 1:1674293/76‑1 (MQ=255) aCGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGttt > 1:897930/1‑76 (MQ=255) cGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTc > 1:1154067/1‑76 (MQ=255) cAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTAtt < 1:457610/76‑1 (MQ=255) gagTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAg < 2:755269/76‑1 (MQ=255) ttGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTg < 1:580379/76‑1 (MQ=255) gcgcGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCta < 2:819493/76‑1 (MQ=255) gcgcGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCta < 1:1580050/76‑1 (MQ=255) gcgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtatt < 1:522619/76‑1 (MQ=255) cgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtatta > 1:1779146/1‑76 (MQ=255) cgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtatta > 1:1331340/1‑76 (MQ=255) cgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtatta > 2:839873/1‑76 (MQ=255) cgTTAATGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtatta < 2:1974779/76‑1 (MQ=255) gTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtattat > 2:419119/1‑76 (MQ=255) ttATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatc > 2:1793671/1‑76 (MQ=255) aTTGAAGCAGCTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatcat < 2:1388406/76‑1 (MQ=255) tGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATcg < 1:1428880/76‑1 (MQ=255) tGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATcg > 2:658936/1‑76 (MQ=255) tGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATcg < 2:1544885/76‑1 (MQ=255) aaGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATcgcg < 2:1186810/76‑1 (MQ=255) aaGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATcgcg > 1:940663/1‑76 (MQ=255) gCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGaa > 1:1488760/1‑76 (MQ=255) gCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGaa < 1:1279685/76‑1 (MQ=255) cAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAAc < 2:1194488/76‑1 (MQ=255) aGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACt > 1:1761214/1‑76 (MQ=255) ggTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTg < 1:221222/76‑1 (MQ=255) aaaCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaaa < 2:1507421/76‑1 (MQ=255) aaaCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaaa < 1:1167654/76‑1 (MQ=255) aaCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaaaa > 2:276692/1‑76 (MQ=255) cATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAAtc > 2:1478603/1‑76 (MQ=255) tttACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAAtctc < 1:1693068/76‑1 (MQ=255) ttACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCa > 2:481329/1‑76 (MQ=255) ttACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCa > 2:130767/1‑76 (MQ=255) ttACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCa > 2:669470/1‑76 (MQ=255) tACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAt > 2:1245197/1‑76 (MQ=255) aCTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAtt > 1:485069/1‑76 (MQ=255) tCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt < 1:324540/76‑1 (MQ=255) cGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTgg < 1:297270/76‑1 (MQ=255) cGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTgg > 1:1177876/1‑76 (MQ=255) gTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGt > 2:961001/1‑76 (MQ=255) cAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGttt > 2:1825022/1‑76 (MQ=255) cAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGttt > 2:955876/1‑76 (MQ=255) cAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGttt < 1:301486/76‑1 (MQ=255) cAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGttt > 2:538336/1‑76 (MQ=255) aGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTc > 2:232468/1‑76 (MQ=255) aGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTc < 2:1075251/76‑1 (MQ=255) ggCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCt > 1:1481497/1‑76 (MQ=255) naaTCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCg > 1:1451172/2‑76 (MQ=255) cAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCg > 1:1262/1‑76 (MQ=255) aTCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCggg < 2:220668/76‑1 (MQ=255) gCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTg > 2:39673/1‑76 (MQ=255) ccGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGa < 2:1171875/76‑1 (MQ=255) cGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGaa > 1:548355/1‑76 (MQ=255) cGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGaa > 1:825693/1‑76 (MQ=255) ggggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAAt < 1:290713/76‑1 (MQ=255) gggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg < 1:288655/76‑1 (MQ=255) gggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg < 1:1745393/76‑1 (MQ=255) | CAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATG > REL606/1290741‑1290885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |