Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,228,283	G→T	P96Q (CCG→CAG)	yraL ←	predicted methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,228,283	0	G	T	100.0%	180.1 / NA	45	P96Q (CCG→CAG)	yraL	predicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/25);  total (20/25)

GTAGCGGCACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCAGCTTTT  >  REL606/3228212‑3228354                                                                        |                                                                        ntAGCGGCACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGt                                                                     >  1:1038790/2‑76 (MQ=255)  tAGCGGCACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGtt                                                                    <  2:912509/76‑1 (MQ=255)   aGCGGCACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTc                                                                   <  1:1379647/76‑1 (MQ=255)      ggCACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCgg                                                                >  2:1826636/1‑76 (MQ=255)         accacGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCAt                                                             <  2:1921720/76‑1 (MQ=255)           cacGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCg                                                           <  1:903882/76‑1 (MQ=255)            acGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCgg                                                          <  1:1546084/76‑1 (MQ=255)             cgcgGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTACGGCATCGGa                                                         >  2:1393628/1‑76 (MQ=255)              gcgGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGaa                                                        <  2:421524/76‑1 (MQ=255)              gcgGATCCCCGCTTCACGGCAGGTACGCACCAGATGGGAGCCAGGATCGTTAATTAGCTGCGGTCCGGCATCGGaa                                                        >  2:1198975/1‑76 (MQ=255)               cgGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGaan                                                       <  1:1915768/76‑2 (MQ=255)               cgGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGaaa                                                       <  2:1508991/76‑1 (MQ=255)                ggATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAAc                                                      <  2:278679/76‑1 (MQ=255)                ggATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAAc                                                      <  1:1634273/76‑1 (MQ=255)                ggATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAAc                                                      <  1:998925/76‑1 (MQ=255)                   tCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAg                                                   >  2:442264/1‑76 (MQ=255)                      ccGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAgcgc                                                <  1:424718/76‑1 (MQ=255)                      ccGCTTCACGGCAGGGACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAgcgc                                                >  2:790645/1‑76 (MQ=255)                       cGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCa                                               <  1:956017/76‑1 (MQ=255)                       cGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCa                                               >  2:1268078/1‑76 (MQ=255)                         ctccACGGCAGGTACGCGCCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAAt                                             <  2:1530013/73‑1 (MQ=255)                         cTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAAt                                             >  2:1403079/1‑76 (MQ=255)                          ttCACGGCAGGTCCGCCCCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCaaag                                            <  2:1115543/76‑3 (MQ=255)                              cGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGtttt                                        >  2:1578419/1‑76 (MQ=255)                              cGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGtttt                                        <  1:1215832/76‑1 (MQ=255)                                gCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGc                                      <  1:1053912/76‑1 (MQ=255)                                  aGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGccc                                    >  1:918806/1‑76 (MQ=255)                                   ggTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGcccc                                   <  2:519130/76‑1 (MQ=255)                                       cGCACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCtt                               >  2:786487/1‑76 (MQ=255)                                         cACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGc                             <  2:150524/76‑1 (MQ=255)                                         cACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGc                             >  1:821242/1‑76 (MQ=255)                                         cACCAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGc                             >  2:167239/1‑76 (MQ=255)                                            cAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGc                          >  1:500619/1‑76 (MQ=255)                                            cAGATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGc                          >  1:347385/1‑76 (MQ=255)                                              gATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCtt                        >  1:1883621/1‑76 (MQ=255)                                              gATGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCtt                        <  2:517233/76‑1 (MQ=255)                                               aTGGTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTc                       <  2:84593/76‑1 (MQ=255)                                                  gTAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGcc                    >  2:1538571/1‑76 (MQ=255)                                                   tAGCCAGGATCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCca                   >  1:1455590/1‑76 (MQ=255)                                                    aGCCAGGATCGTTACTTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCcag                  >  1:1061617/1‑76 (MQ=255)                                                           aTCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGtt           <  2:535124/76‑1 (MQ=255)                                                           aTCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGtt           >  2:1296103/1‑76 (MQ=255)                                                            tCGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGttt          <  2:1279248/76‑1 (MQ=255)                                                             cGTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTc         >  2:182608/1‑76 (MQ=255)                                                              gTTAATTAGCTGCGTTCCGGCATCGGAAACCGGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCa        <  2:1560142/76‑1 (MQ=255)                                                              gTTAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCa        >  2:985538/1‑76 (MQ=255)                                                                tAATTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCAGc      >  1:482438/1‑76 (MQ=255)                                                                  aTTAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCAGCtt    >  2:852164/1‑76 (MQ=255)                                                                    tAGCTGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCAGCtttt  <  2:1038790/76‑1 (MQ=255)                                                                        |                                                                        GTAGCGGCACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCAGCTTTT  >  REL606/3228212‑3228354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑