| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,499,829 | G→T | F325L (TTC→TTA) | glgC ← | glucose‑1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,499,829 | 0 | G | T | 97.8% | 164.5 / ‑2.4 | 45 | F325L (TTC→TTA) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (25/19); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01 | |||||||||||
AACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTCCGG > REL606/3499756‑3499900 | acccGAAGATCACACACCCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTaa < 2:605700/74‑1 (MQ=255)aaCCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGACCCTGCACTaa > 2:1636156/1‑76 (MQ=255) ccGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAAtt > 1:1256437/1‑76 (MQ=255) gagaTCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCg > 2:1585763/1‑76 (MQ=255) gaTCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCt > 1:1605438/1‑76 (MQ=255) caACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTa > 2:1633477/1‑76 (MQ=255) caACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTa > 2:1017965/1‑76 (MQ=255) ccgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGAtt > 2:209598/1‑76 (MQ=255) cgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTc > 2:1641993/1‑76 (MQ=255) aGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAgg < 1:1492953/76‑1 (MQ=255) gTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTTATTGTAggg > 1:1015038/1‑75 (MQ=255) tGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTg > 1:43781/1‑76 (MQ=255) tGAGTTAAGGCTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTg < 2:1390945/76‑1 (MQ=255) tAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAAt < 2:1788850/76‑1 (MQ=255) tAAGGGTCATCCCGCGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAAt < 2:1883453/76‑1 (MQ=255) aaGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTTATTGTAGGTGCGAAtt > 1:1707113/1‑76 (MQ=255) aaGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAAtt > 2:430459/1‑76 (MQ=255) aGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTg > 2:957680/1‑76 (MQ=255) aGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTg < 1:522913/76‑1 (MQ=255) aGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTg > 1:513145/1‑76 (MQ=255) ggTCATCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGCAATGATTCATTGTAGGTGGGAATTGGc > 2:859888/1‑76 (MQ=255) tCCCGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAAtt < 2:1490370/76‑1 (MQ=255) cccGTGGCTACCGGAGCGATCCTGCCCTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTg < 2:255027/76‑1 (MQ=255) ccGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGc > 1:1643224/1‑76 (MQ=255) cGTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTTATTGTAGGGGCGCATTGGCCAATTGCg > 2:465189/1‑76 (MQ=255) gTGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGGAATGATTCATTGTAGGTGCGAAATGGCCAATTGCGa > 2:274170/1‑76 (MQ=255) tGGCTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGAt > 2:1737912/1‑76 (MQ=255) tGGCTACCGGAGCGAACCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGAt < 1:948892/76‑1 (MQ=255) cTACCGGAGCTATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGt < 2:1375587/76‑1 (MQ=255) cTACCGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGt < 1:1107214/76‑1 (MQ=255) tgccGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTa > 2:826767/3‑76 (MQ=255) ccGGAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACa > 2:1279723/1‑76 (MQ=255) cGGAGCGATCGTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACat < 2:1393514/76‑1 (MQ=255) ggAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACata < 1:537225/76‑1 (MQ=255) gAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACatat < 1:798665/76‑1 (MQ=255) gAGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACatat < 1:1655183/76‑1 (MQ=255) aGCGATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATc < 1:1966503/76‑1 (MQ=255) gATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAg < 2:1000138/76‑1 (MQ=255) gATCCTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAg > 2:231808/1‑76 (MQ=255) ccTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTc < 1:31955/76‑1 (MQ=255) ccTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTc > 1:1351120/1‑76 (MQ=255) cTGCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTcc > 2:246661/1‑76 (MQ=255) tGCACTAATTCCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTCCg < 1:845122/76‑1 (MQ=255) gCACTAATTTCGCTGGCGGTAATGGTTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTCCgg < 2:1711802/76‑1 (MQ=255) gCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTCCgg > 2:1170350/1‑76 (MQ=255) gCACTAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTCCgg > 2:338235/1‑76 (MQ=255) gCACGAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTCCgg > 2:1218569/1‑76 (MQ=255) | AACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTCCGG > REL606/3499756‑3499900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |