| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,290,440 | Δ1 bp | coding (405/1014 nt) | hnr → | response regulator of RpoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,290,439 | 0 | A | . | 97.4% | 182.7 / ‑3.4 | 39 | coding (404/1014 nt) | hnr | response regulator of RpoS |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (17/21); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGG > REL606/1290365‑1290510 | tAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTgaggag < 1:552570/76‑2 (MQ=255) aaaGATCTGAATCGCTTGCGCGAGATGGTTTTTGCTTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTgaggaga > 2:1662466/1‑74 (MQ=255) aaaGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTgaggaga > 1:1139056/1‑74 (MQ=255) aaaGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTgaggaga < 2:1104756/76‑3 (MQ=255) aaGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTgaggagag > 1:1349834/1‑73 (MQ=255) gATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTtgagg‑agagga > 2:1142729/1‑76 (MQ=255) gATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTtgagg‑agagga > 1:1109561/1‑76 (MQ=255) aTCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTtgagg‑agaggaa > 2:240022/1‑76 (MQ=255) aTCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTtgagg‑agaggaa < 1:361851/76‑1 (MQ=255) tCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCttt < 2:1019594/76‑1 (MQ=255) cGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑ATAGGAAAGGCtttt < 1:1211872/76‑1 (MQ=255) cGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCtttt < 2:221285/76‑1 (MQ=255) gcgAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGAc > 1:238148/1‑76 (MQ=255) gcgAGATGGTTTTNGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGAc > 1:1242124/1‑76 (MQ=255) cgAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACt < 1:726407/76‑1 (MQ=255) gagaTGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTg > 1:55682/1‑76 (MQ=255) gagaTGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTg > 2:1181683/1‑76 (MQ=255) aTGGTTTTTGCCTGNCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGa > 1:993241/1‑76 (MQ=255) tGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTCTCTCGCGACTGGGAt > 2:1739750/1‑76 (MQ=255) gTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGc > 1:526632/1‑76 (MQ=255) tttGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAAt < 2:313615/76‑1 (MQ=255) ttGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCTACTGGGATGCAATg < 1:978514/76‑1 (MQ=255) gCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGt > 2:1115079/1‑76 (MQ=255) gCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATACAATGGt < 1:1191351/76‑1 (MQ=255) ccTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGACGCAATGGtt > 1:665399/1‑76 (MQ=255) gTCTCTATCCCAGCATGTTTAATTCGCGCGTTGA‑GAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGAt > 2:1663922/1‑76 (MQ=255) tctcTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATa < 2:174330/76‑1 (MQ=255) tctcTATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATa > 1:521949/1‑76 (MQ=255) tctATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAAc < 1:959967/76‑1 (MQ=255) tctATCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAAc > 1:1674839/1‑76 (MQ=255) aTCCCAGCTGGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCt < 2:888345/76‑1 (MQ=255) aTCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCt > 1:1752026/1‑76 (MQ=255) aTCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCt < 2:221406/76‑1 (MQ=255) tCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTg < 1:938328/76‑1 (MQ=255) tCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTg < 1:953709/76‑1 (MQ=255) tCCCAGCATGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTg < 1:953693/76‑1 (MQ=255) aTGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAgc > 1:1085259/1‑76 (MQ=255) aTGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAgc > 2:459897/1‑76 (MQ=255) tGTTTAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAgcg < 1:1724840/76‑1 (MQ=255) tttAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAgcggc < 1:1405500/76‑1 (MQ=255) tAATTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGa < 2:7792/76‑1 (MQ=255) aaTTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGaa > 1:820293/1‑76 (MQ=255) aaTTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGaa < 1:758478/76‑1 (MQ=255) aTTCGCGCGTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGaaa < 2:1111170/76‑1 (MQ=255) ggggcgTTGAGG‑GGAGGAAGGGGTTTTGGGCGGCTGGGATGCAATGGTTGATAACCCTGCCGCGGCGGCGAAatta < 2:1249923/73‑1 (MQ=255) cgcgcgTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAatta < 1:1649276/76‑1 (MQ=255) gcgTTGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAAttatta > 1:1781218/1‑76 (MQ=255) ttGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAg > 2:163504/1‑76 (MQ=255) tGAGG‑AGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAgg > 1:1089058/1‑76 (MQ=255) | TAAAGATCTGAATCGCTTGCGCGAGATGGTTTTTGCCTGTCTCTATCCCAGCATGTTTAATTCGCGCGTTGAGGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGG > REL606/1290365‑1290510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |