| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,298,651 | Δ1 bp | coding (432/1359 nt) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,298,651 | 0 | A | . | 93.5% | 129.2 / 4.0 | 31 | coding (432/1359 nt) | glpT | sn‑glycerol‑3‑phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (12/17); total (13/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
CCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACC > REL606/2298580‑2298720 | ccGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACg < 1:437285/76‑1 (MQ=255) cGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACca < 1:1276543/76‑1 (MQ=255) gTTGTGCGCATAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACcac < 2:1270942/76‑1 (MQ=255) ttGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACcac < 1:1053917/76‑1 (MQ=255) ttGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACcaca < 1:638408/76‑1 (MQ=255) tgtgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCCT‑GTACGACCacac < 1:1222795/76‑1 (MQ=255) gtgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACAcg > 1:1529418/1‑76 (MQ=255) tgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACAcgg > 1:804057/1‑76 (MQ=255) ggcgcACCGCTCCCCGCCGACACAACGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACTcga < 2:1478723/75‑2 (MQ=255) gcgcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACAcggc < 1:394393/76‑1 (MQ=255) gcACACTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACAcggcgg < 2:1813775/76‑1 (MQ=255) aGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCAc < 2:1321849/76‑1 (MQ=255) ttCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCAccc > 2:1472258/1‑76 (MQ=255) tCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCAcccc < 1:615444/76‑1 (MQ=255) ccACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCa > 2:722378/1‑76 (MQ=255) cacTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATcc > 2:513345/1‑76 (MQ=255) gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTg < 2:470824/76‑1 (MQ=255) caATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGgac > 1:1705021/1‑76 (MQ=255) aTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGaacca < 1:526081/76‑1 (MQ=255) tGCCGCCACGTTCTNTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGaaccaa > 1:994701/1‑76 (MQ=255) gccgccACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCCGGaacccac > 1:1152992/1‑76 (MQ=255) ccgccACGTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGaaccaacc < 2:384412/76‑1 (MQ=255) gTTCTTTCTGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAgg < 2:674481/76‑1 (MQ=255) ttctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaaca > 1:1117796/1‑76 (MQ=255) ttctGCGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaacaa < 1:200123/76‑1 (MQ=255) tgctGCGACCACCAGTGCGCCAT‑GTCCGACCACACGGCGGCCTCCCCATCCCCTGGAACCAACCGCAGAGGaacaa < 2:196995/74‑1 (MQ=255) gcgcCCACCAGTGCACCAT‑GTACGACCACACGGCGTCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGt < 1:512941/76‑1 (MQ=255) cGACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCCTCCCCTGGAACCAACCGCAGAGGAACAACAgaa > 2:348532/1‑74 (MQ=255) gACCACCAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTAc > 1:1308743/1‑76 (MQ=255) accaccAGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACa < 2:349434/76‑1 (MQ=255) caGTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACAt < 1:347483/76‑1 (MQ=255) gTGCATCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCa < 2:1817038/76‑1 (MQ=255) gTGCACCAT‑GTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCa > 1:1400762/1‑76 (MQ=255) tGCACCAT‑GTACGNCCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCAc > 1:1234184/1‑76 (MQ=255) gCACCAT‑GTACGGCCACACGGCGCCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCAcc < 2:921868/76‑1 (MQ=255) | CCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACC > REL606/2298580‑2298720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |