Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	474,424	A→T	D294E (GAT→GAA)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	474,424	0	A	T	100.0%	155.1 / NA	41	D294E (GAT→GAA)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/22);  total (19/22)

TAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGG  >  REL606/474352‑474493                                                                         |                                                                      tAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCa                                                                    <  1:245738/76‑1 (MQ=255) tAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCa                                                                    >  2:687758/1‑76 (MQ=255)  aaCGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCaa                                                                   >  2:940665/1‑76 (MQ=255)     gccTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAaaca                                                                <  2:1420528/74‑1 (MQ=255)     gACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGGATCAGCATTAACGGTTCAaaca                                                                >  1:1079370/1‑76 (MQ=255)     gACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCCGAATCAGAATTAACGGTTCAaaca                                                                <  1:301793/76‑1 (MQ=255)      aCTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAaacaa                                                               >  2:1833792/1‑76 (MQ=255)        ttACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAacaaca                                                             <  1:962688/76‑1 (MQ=255)        ttACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAacaaca                                                             >  1:1779232/1‑76 (MQ=255)            cAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATccc                                                         <  2:1575462/76‑1 (MQ=255)            cAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATccc                                                         <  1:1455824/76‑1 (MQ=255)                  gAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCTACGga                                                   >  2:1795021/1‑76 (MQ=255)                  gAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGga                                                   >  1:1678555/1‑76 (MQ=255)                  gAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGGTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGga                                                   >  1:1218700/1‑76 (MQ=255)                  aaataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGga                                                   <  2:534825/75‑1 (MQ=255)                   aataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGgag                                                  >  1:87481/1‑76 (MQ=255)                     taatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGAc                                                <  1:784443/76‑1 (MQ=255)                       atCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACaa                                              <  2:1704063/76‑1 (MQ=255)                          gCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACaaaaa                                           <  1:945548/76‑1 (MQ=255)                          gCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACaaaaa                                           >  1:1244821/1‑76 (MQ=255)                            cAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAAc                                         <  1:1733817/76‑1 (MQ=255)                              ttctCGCCAGCACCGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAg                                       >  2:1469817/4‑76 (MQ=255)                                gTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCa                                     >  2:509616/1‑76 (MQ=255)                                  cGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCAcc                                   >  2:363457/1‑76 (MQ=255)                                   gCCAGCTCTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCg                                  <  2:1464087/76‑1 (MQ=255)                                   gCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCg                                  >  1:1055439/1‑76 (MQ=255)                                   gCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCg                                  <  2:857297/76‑1 (MQ=255)                                   gCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCg                                  <  1:709896/76‑1 (MQ=255)                                     cAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAGAAAACAGCACCGCa                                <  1:1003457/76‑1 (MQ=255)                                        cACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAAc                             <  2:1818419/76‑1 (MQ=255)                                            gCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGt                         >  2:1062602/1‑76 (MQ=255)                                              cAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTcg                       >  2:1613793/1‑76 (MQ=255)                                              cAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTcg                       <  2:1693432/76‑1 (MQ=255)                                              cAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTcg                       >  1:1217459/1‑76 (MQ=255)                                               aGCGGTTGCTGAATCAAAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCGCCGCAAACGCGTcgc                      <  2:1750591/76‑1 (MQ=255)                                                       cTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACTCGTCGCGCAATGGc              <  1:985185/76‑1 (MQ=255)                                                        tGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGCCAAAAAACAGCACCGCAAACGCGTCGCGCAATgcag             >  1:1422370/1‑73 (MQ=255)                                                           aTCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCg          <  1:686668/76‑1 (MQ=255)                                                             cAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTa        >  2:1542336/1‑76 (MQ=255)                                                              aGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTAt       <  2:170170/76‑1 (MQ=255)                                                              aGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTAt       >  1:1783122/1‑76 (MQ=255)                                                                aaTTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCg     <  2:536343/76‑1 (MQ=255)                                                                   tAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTgg  >  1:5028/1‑76 (MQ=255)                                                                         |                                                                      TAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGG  >  REL606/474352‑474493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑