Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,131,466	(A)9→8	intergenic (‑125/+175)	gatY ← / ← fbaB	D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,131,458	0	A	.	100.0%	142.6 / NA	29	intergenic (‑117/+183)	gatY/fbaB	D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (22/7);  total (22/7)

AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACAT  >  REL606/2131383‑2131530                                                                            |                                                                          aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa                                                                           >  2:1480958/1‑76 (MQ=255)   atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa                                                                         <  1:1276139/76‑1 (MQ=255)     gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa                                                                       <  2:1485257/76‑1 (MQ=255)         tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac                                                                   >  2:1479134/1‑75 (MQ=255)         tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac                                                                   <  1:1353140/76‑2 (MQ=255)               tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc                                                            >  2:934848/1‑76 (MQ=255)                  aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑g                                                        <  2:1500799/76‑1 (MQ=255)                    gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa                                                      <  1:186511/76‑1 (MQ=255)                    gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa                                                      >  1:602441/1‑76 (MQ=255)                      cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTCCC‑GGAggg                                                   <  2:480935/76‑1 (MQ=255)                        agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGa                                                  <  2:504504/76‑1 (MQ=255)                          aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAg                                                >  1:183699/1‑76 (MQ=255)                          aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAg                                                >  1:776505/1‑76 (MQ=255)                           tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGc                                               >  1:423420/1‑76 (MQ=255)                             aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCg                                             <  2:74412/76‑1 (MQ=255)                              cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGc                                            >  1:222471/1‑76 (MQ=255)                                  cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGcaaca                                        >  2:910591/1‑76 (MQ=255)                                  cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGcaaca                                        >  1:877912/1‑76 (MQ=255)                                  cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGcaaca                                        >  1:18182/1‑76 (MQ=255)                                    aaaaaaCAAAAATATAAAAATAAAAAGAGAAATAAAAGG‑AAAAAAAACCGGCCTCCC‑GGAGGGAAGCCGCAACaaa                                      <  2:950918/75‑1 (MQ=255)                                      aaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGCAAGCCGCAACAAAga                                    >  2:609161/1‑75 (MQ=255)                                         aCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTgcg                                 >  1:310311/1‑76 (MQ=255)                                                 atGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGttt                         >  1:1166757/1‑76 (MQ=255)                                                  aaaaaaTAAAAAGAGAAAAAAAAGG‑AAAAAAAACCGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGtttt                        <  1:695344/74‑1 (MQ=255)                                                    aaaaTTAATAGAGGAATGAAAGG‑AAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt                      <  2:1119771/76‑1 (MQ=255)                                                      agttattaGGGGATTGAATGGC‑ATAATAATCGGCCGCCG‑GGAGTGAAGCCGCAACAAAGTGCGCGTGGTTTTATGc                    <  1:266744/74‑1 (MQ=255)                                                       aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa                   <  2:958943/76‑1 (MQ=255)                                                        ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg                  >  1:1052637/1‑76 (MQ=255)                                                        ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGAGCGCGCGGTTTTATGCAg                  >  1:501185/1‑76 (MQ=255)                                                         tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg                 >  2:207899/1‑76 (MQ=255)                                                          aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa                >  1:1161457/1‑76 (MQ=255)                                                           aaagagGAAGGAAAGGC‑AAAAAAAGCGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt               <  2:1539931/74‑1 (MQ=255)                                                            tAGAGAAAGGAAA‑GAAAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg              <  2:1515579/76‑1 (MQ=255)                                                              gagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg            >  2:1062630/1‑76 (MQ=255)                                                              gagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg            >  1:622821/1‑76 (MQ=255)                                                               gagaaagaaaGGGAAAAAAAAGCGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg            <  1:273210/74‑1 (MQ=255)                                                               agGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg           >  2:933361/1‑76 (MQ=255)                                                               agGAATAAAAGGC‑AAAAAAACCGGCCCCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg           <  2:310311/76‑1 (MQ=255)                                                                  aaTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt        >  1:853562/1‑76 (MQ=255)                                                                   aggAAAGG‑AAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa       <  2:1268388/74‑1 (MQ=255)                                                                   aTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa       >  1:1599156/1‑76 (MQ=255)                                                                      aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc    >  2:713429/1‑76 (MQ=255)                                                                          aaaaaaaaaaaCCGCCCCCCGGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACat  <  1:62303/74‑1 (MQ=37)                                                                            |                                                                          AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACAT  >  REL606/2131383‑2131530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑