Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,497,251	G→A	S262F (TCC→TTC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,497,251	0	G	A	93.3%	145.8 / 3.1	45	S262F (TCC→TTC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (18/24);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01

TTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTA  >  REL606/3497178‑3497319                                                                          |                                                                     ttCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAg                                                                    <  2:260190/76‑1 (MQ=255)  tCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGt                                                                   >  1:959162/1‑76 (MQ=255)    cTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGGCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTgg                                                                 >  2:230303/1‑76 (MQ=255)     tGACGCAGGCGCAGCTCCCGCCCGGAGTAGGTGGAGTCATCCGAATACAGTACGCGAGATACGTTCTCGAAGTGGt                                                                <  2:1386619/76‑1 (MQ=255)     tGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGt                                                                <  1:1176037/76‑1 (MQ=255)       aCGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGttt                                                              <  1:1318870/76‑1 (MQ=255)             gcgcAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCt                                                        <  1:894914/76‑1 (MQ=255)              cgcAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCtt                                                       <  2:1544190/76‑1 (MQ=255)                cAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTcc                                                     <  2:169838/76‑1 (MQ=255)                cAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTcc                                                     <  2:292576/76‑1 (MQ=255)                cAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTcc                                                     >  1:915362/1‑76 (MQ=255)                 aGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCa                                                    <  2:1395088/76‑1 (MQ=255)                  gCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCAc                                                   >  1:1028589/1‑76 (MQ=255)                  gCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCAc                                                   >  1:823309/1‑76 (MQ=255)                       cGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCcg                                              <  1:71552/76‑1 (MQ=255)                       cGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCcg                                              >  2:337845/1‑76 (MQ=255)                             gAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAagt                                        <  1:1414574/76‑1 (MQ=255)                              aGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAagta                                       <  1:1261968/76‑1 (MQ=255)                              aGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAagta                                       >  1:1065595/1‑76 (MQ=255)                              aGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCATCCACTGCCGCGAagta                                       >  1:692037/1‑76 (MQ=255)                                  ggTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCa                                   <  1:215582/76‑1 (MQ=255)                                    tGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCAcc                                 >  1:668229/1‑76 (MQ=255)                                     ggAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGCAGTAGTCAccc                                >  1:358624/1‑76 (MQ=255)                                     ggAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCAccc                                >  2:483221/1‑76 (MQ=255)                                     ggAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCAccc                                <  2:1243687/76‑1 (MQ=255)                                      gAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCt                               <  1:966217/76‑1 (MQ=255)                                      gAGTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCt                               <  1:612777/76‑1 (MQ=255)                                        gTCATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTgg                             >  2:792652/1‑76 (MQ=255)                                         tCATCCGGATACAGTACGCTAGATACGTTCTCGAATTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGt                            >  1:633369/1‑76 (MQ=255)                                          cATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGtt                           <  2:1110972/76‑1 (MQ=255)                                          cATCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGtt                           <  1:667850/76‑1 (MQ=255)                                           aTCCGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTg                          <  2:1198533/76‑1 (MQ=255)                                             ccGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGaa                        >  1:422470/1‑76 (MQ=255)                                              cGGATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAAt                       >  2:919317/1‑76 (MQ=255)                                                gATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAAttt                     <  1:932982/76‑1 (MQ=255)                                                gATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAAttt                     <  2:1310774/76‑1 (MQ=255)                                                gATACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAAttt                     <  1:898367/76‑1 (MQ=255)                                                 aTACAGTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTa                    <  2:1471457/76‑1 (MQ=255)                                                      gTACGCGAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAg               >  2:1085119/1‑76 (MQ=255)                                                         cgcgAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGtt            <  2:1407195/76‑1 (MQ=255)                                                         cgcgAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGtt            >  2:403461/1‑76 (MQ=255)                                                          gcgAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTa           <  1:585321/76‑1 (MQ=255)                                                          gcgAGATACGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTa           <  1:32145/76‑1 (MQ=255)                                                                  cGTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCt   <  2:987560/76‑1 (MQ=255)                                                                   gTTCTCGAAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTa  >  1:270398/1‑76 (MQ=255)                                                                          |                                                                     TTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTA  >  REL606/3497178‑3497319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑