Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,032,114	A→G	R228R (CGT→CGC)	manB ←	Phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,032,114	0	A	G	100.0%	50.8 / NA	16	R228R (CGT→CGC)	manB	Phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/6);  total (10/6)

AAACAGGAAACAGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTACGGGTGTCGTCGCGGCATTCCGGCAGC  >  REL606/2032042‑2032141                                                                         |                            aaaCAGGAAACAGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCgg                          >  1:326680/1‑76 (MQ=255)  aaCAGGAAACAGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCggg                         <  1:824277/76‑1 (MQ=255)     aGGAAACAGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGgtgt                      >  1:1352871/1‑76 (MQ=255)     aGGAAACAGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGgtgt                      >  2:693120/1‑76 (MQ=255)      ggAAACAGCGGTTAAAATCGCCATCAAAGGCAATGCCCATATCGGCGCCGTGTTTGATGCCCGCATGGCGGGTgtc                     >  2:1559016/1‑76 (MQ=14)      ggAAACAGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTgtc                     >  1:389644/1‑76 (MQ=255)      ggAAACAGCGGTCAAAATCGCCATCAAAGGCAATGACCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTgtc                     >  2:1451935/1‑76 (MQ=255)         aaCAGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTgtcgtc                  >  2:848218/1‑76 (MQ=255)            aGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTcgcg               <  2:1308469/76‑1 (MQ=255)            aGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTcgcg               <  1:1573317/76‑1 (MQ=255)              cGGTCAAAATCGCCATCAAAGGCAACGCCCATATCCGCGCCGTGTTCGNTGACAGAATAGCCGGCNTCGTcgcgac             >  1:1323098/1‑74 (MQ=9)               ggTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCa            <  1:1562705/76‑1 (MQ=255)                gTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCAt           >  1:1503397/1‑76 (MQ=25)                   aaaaTCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTcc        >  2:1505970/1‑76 (MQ=21)                     aaTCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCgg      >  2:661017/1‑76 (MQ=21)                      aTCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATGGCGGGTGTCGTCGCGGCATTCCGgc     <  2:1416/76‑1 (MQ=255)                        cGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGgcag   >  1:915726/1‑76 (MQ=17)                         gCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTGCGGGTGTCGTCGCGGCATTCCGgcagc  <  2:778680/76‑1 (MQ=14)                                                                         |                            AAACAGGAAACAGCGGTCAAAATCGCCATCAAAGGCAATGCCCATATCCGCGCCGTGTTTGATGACCGCATTACGGGTGTCGTCGCGGCATTCCGGCAGC  >  REL606/2032042‑2032141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑