| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,251,818 | C→T | A315T (GCG→ACG) | nusA ← | transcription elongation factor NusA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,251,818 | 0 | C | T | 91.2% | 109.0 / ‑0.5 | 34 | A315T (GCG→ACG) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (11/20); total (13/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTG > REL606/3251750‑3251889 | gTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTa > 1:940669/1‑76 (MQ=255)gTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTa < 1:295215/76‑1 (MQ=255) gAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCg > 1:1576537/1‑76 (MQ=255) tCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCtt < 1:1426896/76‑1 (MQ=255) cccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCCTc > 1:180802/1‑76 (MQ=255) ccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCa < 2:1273813/76‑1 (MQ=255) ccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCa < 1:246326/76‑1 (MQ=255) ccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCa < 1:526906/76‑1 (MQ=255) gCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACNGGCTTCAACggag < 1:1078472/76‑3 (MQ=255) gCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCg < 1:813284/76‑1 (MQ=255) cTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCCTCAACGGCGa > 2:833483/1‑76 (MQ=255) tCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGat < 1:1046418/76‑1 (MQ=255) aGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGGGCCAGGCTACCGGCTTCAACGGGGatat > 1:1517224/1‑76 (MQ=255) cTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATcc < 1:422724/76‑1 (MQ=255) aaGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTCACGGCGATATCCATggg > 2:261229/1‑75 (MQ=255) aaGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGgt < 2:986768/76‑1 (MQ=255) cGCACGTCCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTAt < 2:421680/76‑1 (MQ=255) gCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATc > 2:916356/1‑76 (MQ=255) cACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTAACGGCGAGATCCATGGGGGGGTTATCt > 2:544220/1‑76 (MQ=255) aCGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 1:1121940/76‑1 (MQ=255) aCGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt > 1:630190/1‑76 (MQ=255) gTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa < 1:1426620/76‑1 (MQ=255) gTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCCACGGCGGTATCCCTGGTGTGTTTATCttta > 2:360106/1‑74 (MQ=255) cTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATcc < 2:1465413/76‑1 (MQ=255) gACCGTTACGGCCAGTCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccac > 1:863436/1‑76 (MQ=255) gACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccac > 2:752563/1‑76 (MQ=255) tACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGAt < 1:1015642/76‑1 (MQ=255) ggCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGa < 1:57217/76‑1 (MQ=255) gCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGaa < 1:1590854/76‑1 (MQ=255) ccAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAg < 2:764649/76‑1 (MQ=255) aaTCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCa > 1:749179/1‑76 (MQ=255) aaTCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCa > 1:596307/1‑76 (MQ=255) ccTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTc < 1:341910/76‑1 (MQ=255) cTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCt < 1:255291/76‑1 (MQ=255) tGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTg < 1:1555623/76‑1 (MQ=255) cgcgtCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTg < 2:416072/75‑1 (MQ=255) | GTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTG > REL606/3251750‑3251889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |