| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,414,644 | A→G | Y207C (TAC→TGC) | crp → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,414,644 | 0 | A | G | 97.6% | 165.1 / ‑4.3 | 42 | Y207C (TAC→TGC) | crp | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (27/14); total (27/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGC > REL606/3414572‑3414714 | ccGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCgg > 2:1469687/1‑76 (MQ=255) gTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCa > 1:51281/1‑76 (MQ=255) tGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCAc > 1:855302/1‑76 (MQ=255) ggACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTc < 1:111675/76‑1 (MQ=255) aTTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAAt > 1:259/1‑76 (MQ=255) aTTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAAt > 2:455004/1‑76 (MQ=255) aTTCTGAAGATGCTGGAAGATCAGAACCTGATCTCAGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAAt > 2:1422041/1‑76 (MQ=255) tCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATcc < 1:94050/76‑1 (MQ=255) tCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGGAAAACCATCGTCGTTTGCGGCACTCGTTAATcc > 1:148708/1‑76 (MQ=255) tGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCg > 2:1358369/1‑76 (MQ=255) aaGGTGCTGGAAGATCAGATCCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTc < 2:310480/76‑1 (MQ=255) aGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAGCCATCGTCGTTTACGGCACTCGTTAATCCCGTCg < 2:1295173/76‑1 (MQ=255) aGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCg > 1:303558/1‑76 (MQ=255) ggAAGATCAGAACNTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGgc > 1:1080771/1‑76 (MQ=255) ggAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGgc > 2:1527961/1‑76 (MQ=255) aaGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGgcgc > 2:566072/1‑76 (MQ=255) aaGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGgcgc > 2:130159/1‑76 (MQ=255) gATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGt < 1:1447105/76‑1 (MQ=255) cAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTAc > 1:1323562/1‑76 (MQ=255) cAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTAc < 2:565055/76‑1 (MQ=255) aaCCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTg < 2:303558/76‑1 (MQ=255) aaCCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTg < 2:543058/76‑1 (MQ=255) aaCCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTg < 2:1355766/76‑1 (MQ=255) aaCCTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTg < 1:1259106/76‑1 (MQ=255) ccTGATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGt < 1:1572335/76‑1 (MQ=255) gATCTCCGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCCCTACCTGGTAgc > 1:1297125/1‑76 (MQ=255) ccGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGcc > 2:1503202/1‑76 (MQ=255) ccGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGcc > 2:1550407/1‑76 (MQ=255) ccGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGGAGCGCGcc > 2:987443/1‑76 (MQ=255) cGCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCa > 2:422367/1‑76 (MQ=255) gCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAt > 1:252124/1‑76 (MQ=255) gCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAt > 2:1528429/1‑76 (MQ=255) gCACACGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAt > 2:643545/1‑76 (MQ=255) cacaCGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAtt < 1:668911/76‑1 (MQ=255) cacaCGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCCCGCCCtt > 1:669488/1‑76 (MQ=255) acacGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAttt > 2:459480/1‑76 (MQ=255) cacGGTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAtttt < 1:1065443/76‑1 (MQ=255) ggTAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGtt < 2:1158505/76‑1 (MQ=255) tAAAACCATCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGGGGGGCCTTTTGTTTc > 2:518397/1‑76 (MQ=255) aTCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAt < 2:1529197/76‑1 (MQ=255) aTCGTCGTTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAt > 2:507621/1‑76 (MQ=255) cgtTTGCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGCAGCGCGCCCTTTTGTTTCCCCCGATGGGgc > 1:1263395/1‑76 (MQ=255) | CCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGC > REL606/3414572‑3414714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |