| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,270 | C→T | E346K (GAG→AAG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,270 | 0 | C | T | 92.9% | 88.6 / ‑0.5 | 29 | E346K (GAG→AAG) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (15/11); total (16/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
AGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGA > REL606/474197‑474341 | aGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTAc > 2:54871/1‑76 (MQ=255) ccAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAAt < 1:823600/76‑1 (MQ=255) gTTTTGTCCGTCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCCGGAAAAACGCGCACTAACCAATCTgc > 1:234387/1‑76 (MQ=255) gTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTgc > 1:825754/1‑76 (MQ=255) gTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTgc < 1:4315/76‑1 (MQ=255) tGTCCGGCCGGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCa < 2:713137/76‑1 (MQ=255) gTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAg > 1:883024/1‑76 (MQ=255) gCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTgg < 2:909496/76‑1 (MQ=255) gCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTgg > 2:191915/1‑76 (MQ=255) ccGGCATGAAATTCAATTCCATTCCCAGCCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTggcggc < 2:671408/75‑1 (MQ=255) tcAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGAt > 1:663446/2‑76 (MQ=255) aaTTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTaa < 2:1027037/76‑1 (MQ=255) aTTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGTTGGTTAAt > 2:447177/1‑76 (MQ=255) aTGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGGCga > 2:457638/1‑75 (MQ=255) aTGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGt > 2:56598/1‑76 (MQ=255) ccATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACg < 1:1178921/76‑1 (MQ=255) cccAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTggg < 1:702166/76‑1 (MQ=255) cccAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTggg > 2:314188/1‑76 (MQ=255) cccAGTCCCGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGGTAATGCCGTACGTTggg > 1:372048/1‑76 (MQ=255) ccGCCAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGAcc > 2:163277/1‑76 (MQ=255) cAGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAAc < 1:1043438/76‑1 (MQ=255) aGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACa < 1:1196251/76‑1 (MQ=255) aGGATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACa < 1:251190/76‑1 (MQ=255) ggATAAACGCGAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAg < 1:1186749/76‑1 (MQ=255) cgcgAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCAcc > 2:729270/1‑76 (MQ=255) naaCTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGGGACCAAACAGTCGCACCAgg > 1:462509/2‑76 (MQ=255) gAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAgg > 1:918011/1‑76 (MQ=255) gAACTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAgg < 1:528189/76‑1 (MQ=255) aaCTTACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGa > 1:725760/1‑76 (MQ=255) | AGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGA > REL606/474197‑474341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |