| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,886,204 | G→A | intergenic (‑34/+635) | yobF ← / ← yebO | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,886,204 | 0 | G | A | 88.9% | 175.4 / 7.9 | 54 | intergenic (‑34/+635) | yobF/yebO | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (25/23); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCG > REL606/1886131‑1886272 | cgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTAtt > 2:494730/1‑76 (MQ=255) gtgtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTa > 1:951588/1‑76 (MQ=255) tgtTTACTCAGGANTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCtattat > 1:854508/1‑76 (MQ=255) gtTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATc > 2:62271/1‑76 (MQ=255) tACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATcaca > 1:344017/1‑76 (MQ=255) tACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATcaca > 1:290209/1‑76 (MQ=255) aCTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACAt > 1:824698/1‑76 (MQ=255) cAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACa < 1:918379/76‑1 (MQ=255) cAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACa < 2:961770/76‑1 (MQ=255) ggACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAAc < 2:848035/76‑1 (MQ=255) ggACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAAc < 2:70974/76‑1 (MQ=255) cttcttTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGtt < 1:389511/76‑1 (MQ=255) cttcttTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGtt < 1:674427/76‑1 (MQ=255) tcttTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTaa < 1:804104/76‑1 (MQ=255) cttTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAAt < 2:205490/76‑1 (MQ=255) cttTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAAt > 2:412645/1‑76 (MQ=255) tttACTGAAAATGCCACACATAAACAGAACTGTACTTCGTTTAACCCGAAATCTATTATCACATACAACGTTAAtt > 2:1141209/1‑76 (MQ=255) tttACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAAtt < 2:165176/76‑1 (MQ=255) ttACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTa > 1:175688/1‑76 (MQ=255) tACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATCCAACGCTCATTAt > 1:151128/1‑76 (MQ=255) tACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTAt > 1:1050029/1‑76 (MQ=255) tACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTAt < 2:350017/76‑1 (MQ=255) tACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTAt < 2:1066702/76‑1 (MQ=255) aCTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATg > 1:638244/1‑76 (MQ=255) aCTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATg > 1:1191176/1‑76 (MQ=255) aCTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATg < 1:417789/76‑1 (MQ=255) cTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATgg < 1:1004347/76‑1 (MQ=255) tGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGc < 2:42706/76‑1 (MQ=255) gAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCa > 2:667937/1‑76 (MQ=255) aaaaTGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAATGTTAATTATGGCaa < 1:678789/76‑1 (MQ=255) aaaaTGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCaa > 1:360391/1‑76 (MQ=255) aaaTGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAg < 1:54356/76‑1 (MQ=255) gCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCAt > 1:149802/1‑76 (MQ=255) cacaTAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCAttttt > 2:151453/1‑76 (MQ=255) cacaTAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCAttttt > 2:402098/1‑76 (MQ=255) caTAAACAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTaa < 1:1126884/76‑1 (MQ=255) aTAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaaa > 2:53017/1‑76 (MQ=255) cAGAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGt < 2:32883/76‑1 (MQ=255) gAACTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGtct < 2:560310/76‑1 (MQ=255) aCTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCg > 2:834724/1‑76 (MQ=255) aCTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCg < 1:192771/76‑1 (MQ=255) cTGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGa > 2:281182/1‑76 (MQ=255) tGTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGAt > 1:539678/1‑76 (MQ=255) gTACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATc > 1:592566/1‑76 (MQ=255) tACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCa < 2:635781/76‑1 (MQ=255) tACCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCa < 1:310870/76‑1 (MQ=255) aCCTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAg > 1:1088362/1‑76 (MQ=255) cTCGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGAc > 2:1056741/1‑76 (MQ=255) ccGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACg < 2:394845/75‑1 (MQ=255) cGTTTAACCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGc > 1:995875/1‑76 (MQ=255) aaCCCGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACaaa > 1:1181976/1‑76 (MQ=255) cccGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAAtc < 1:1105474/76‑1 (MQ=255) cccGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAAtc < 1:802364/76‑1 (MQ=255) cGAAATCTATTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAAtctc > 2:1170635/1‑76 (MQ=255) gAAATCTATTATCACATACAACGTTAATTATGGCATGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCg > 2:1133784/1‑76 (MQ=255) | CGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCG > REL606/1886131‑1886272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |