Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,519,775	T→C	T272A (ACA→GCA)	dapA ←	dihydrodipicolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,519,775	0	T	C	100.0%	109.4 / NA	29	T272A (ACA→GCA)	dapA	dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

TAAACTTTACAGCAAACCGGCATGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGTCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCTTACATGCCCATTTCACCGGGATTGG  >  REL606/2519704‑2519844                                                                        |                                                                      tAAACTTTACAGCAAACCGGCATGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCAtt                                                                   <  2:423668/76‑1 (MQ=255)    aCTTTACAGCAAACCGGCATGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGc                                                                >  1:497862/1‑76 (MQ=255)     cTTTACAGCAAACCGGCATGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCa                                                               >  1:991273/1‑76 (MQ=255)        tACAGCAAACCGGCATGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAggg                                                            >  1:596133/1‑75 (MQ=255)            gCAAACCGGCATGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAg                                                        <  1:745035/76‑1 (MQ=255)             cAAACCGGCATGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCCCAGc                                                       >  2:659546/1‑76 (MQ=255)               aaCCGGCATGCTTAGGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGt                                                     <  1:641621/76‑1 (MQ=255)                     cATGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCCGCGTATCggc                                               >  1:944157/1‑75 (MQ=255)                      aTGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGGGCGGCGGATCgggg                                              >  1:945960/1‑74 (MQ=255)                       tGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCCGCGTgtcggtcg                                             >  2:820655/1‑76 (MQ=255)                        gCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGgcgc                                            >  1:275141/1‑76 (MQ=255)                        gCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGTCGc                                            <  1:1138142/76‑1 (MQ=255)                           tAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGGATCGGTCGCcac                                         >  1:1093034/1‑76 (MQ=255)                             aGCGCCGCTCTGAACGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGGGGAGCGGATCGGGCGCCCCaa                                       >  1:840835/1‑76 (MQ=255)                                gccgcTCTCACCCTCTCCCGCCCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGAc                                    <  1:309158/76‑1 (MQ=255)                                    ctctGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGGGCAGCGTATCGGCCGCCCCCAGAccccg                                >  1:1106102/1‑74 (MQ=255)                                    ctctGACCGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAg                                <  1:400948/76‑1 (MQ=255)                                          ccGTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCtt                          <  1:367815/76‑1 (MQ=255)                                            gTCTCACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCTTAc                        <  1:372359/76‑1 (MQ=255)                                             tctcACGACCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCTTACa                       <  1:21676/76‑1 (MQ=255)                                                    aCCACTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGGATCGGGCGCCACAAGACCCAGATCCTTACAAGCCCAt                >  1:989211/1‑76 (MQ=255)                                                       agtGTCGGTGATTGGTGCCATTGGGAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCTTACATGCCCATTTc             <  1:742092/74‑1 (MQ=255)                                                       aCTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCTTACATGCCCATTTc             <  1:764523/76‑1 (MQ=255)                                                       aCTGTCGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCTTACATGCCCATTTc             >  2:61005/1‑76 (MQ=255)                                                          gTCGGTGATTGGTGCCATTGGCAGGGGCAGCGTATCGGTCGCCACAAGACCCAGTTCCCTACATGCCCATTTCAcc          >  1:229276/1‑76 (MQ=255)                                                            cGGTGATTGGTGCCATTGGCAGGCGCAGCGTATCGGGCGGCACAAGACCGAGTTCATTCCATGCCCCCTTCCCCgg        >  2:113318/1‑76 (MQ=255)                                                               tGATTGGTGCCATTGGCAGGCGCCGCGTATCGGTCGCCACAAGACCCCGTTCCTTACATTCCCATTTCACCggggt     >  2:1024306/1‑74 (MQ=255)                                                                 aTTGGTGCCATTGGCAGGCGCAGCGGATCGGGCGCCACAAGACCCAGTTCCTTACATGCCCATTTTCCCGgggttg   >  1:172272/1‑76 (MQ=255)                                                                  ttGGTGCCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCTTACATGCCCATTTCACCGggattgg  <  1:825112/76‑1 (MQ=255)                                                                  ttGGTGCCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCTTACATGCCCATTTCACCGggattgg  <  1:641751/76‑1 (MQ=255)                                                                        |                                                                      TAAACTTTACAGCAAACCGGCATGCTTAAGCGCCGCTCTGACCGTCTCACGACCACTGTCGGTGATTGGTGTCATTGGCAGGCGCAGCGTATCGGTCGCCACAAGACCCAGTTCCTTACATGCCCATTTCACCGGGATTGG  >  REL606/2519704‑2519844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑