Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,014,857	T→G	L93R (CTG→CGG)	ECB_02816 →	KpsD protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,014,857	0	T	G	100.0%	107.0 / NA	27	L93R (CTG→CGG)	ECB_02816	KpsD protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/17);  total (10/17)

TGGTGCTCAACTTTTCAACGGCACCAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCTGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAGAT  >  REL606/3014786‑3014923                                                                        |                                                                   tGGTGCTCAACTTTTCAACGGCACCAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAAt                                                                >  1:106346/1‑76 (MQ=255) tGGTGCTCAACTTTTCAACGGCACCAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAAt                                                                <  1:779626/76‑1 (MQ=255) tGGAGCTCAACTTTTCAACGGCACCAGCGCGGTTAGCGGCACGACGGAAGGATTCAACCCTGACTATATTCGGAAt                                                                >  2:927797/1‑76 (MQ=255)   gTGCTCAACTTTTCAACGGCACCAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATcc                                                              >  1:510593/1‑76 (MQ=255)      cTCAACTTTTCAACGGCACCAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCggg                                                           <  1:1079048/76‑1 (MQ=255)          aCTTTTCAACGGCACCAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGAt                                                       >  1:1206392/1‑76 (MQ=255)             tttCAACGGCACCAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGc                                                    <  2:361210/76‑1 (MQ=255)              ttCAACGGCACCAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGCa                                                   <  2:917942/76‑1 (MQ=255)                      ccccAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGtt                                           <  1:598608/74‑1 (MQ=255)                        ccAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCg                                         <  1:1255269/76‑1 (MQ=255)                         cAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGc                                        >  2:1209449/1‑76 (MQ=255)                               ggATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTgg                                  <  1:1095732/76‑1 (MQ=255)                                      ggTGCGGCGGTAGGATTCTACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGt                           <  1:37496/76‑1 (MQ=255)                                       gTGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGtt                          <  1:348527/76‑1 (MQ=255)                                        tGCGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTc                         >  1:973957/1‑76 (MQ=255)                                          cGACGGTAGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCAc                       <  1:279601/76‑1 (MQ=255)                                                 aGGATTCAACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGAt                >  2:808072/1‑76 (MQ=255)                                                    aTTCAACCCTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGt             <  2:636429/76‑1 (MQ=255)                                                    aTTCAACCCGGACTATAGTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGt             <  1:741490/76‑1 (MQ=255)                                                           ccTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTAc      <  1:111350/76‑1 (MQ=255)                                                           ccTGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTAc      <  1:36136/76‑1 (MQ=255)                                                             tGACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAg    <  1:514259/76‑1 (MQ=255)                                                              taCTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAGa   >  2:883175/2‑76 (MQ=255)                                                              gACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAGa   <  1:516680/76‑1 (MQ=255)                                                              gACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAGa   >  2:538504/1‑76 (MQ=255)                                                              gACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAGa   >  1:364396/1‑76 (MQ=255)                                                              gACTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAGa   <  1:170426/76‑1 (MQ=255)                                                               aCTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAGAt  <  2:279215/76‑1 (MQ=255)                                                               aCTATATTCGGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAGAt  >  2:612800/1‑76 (MQ=255)                                                                        |                                                                   TGGTGCTCAACTTTTCAACGGCACCAGCGCGGATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCTGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGCGTTCACCTTTGATGGTGCATTACAGAT  >  REL606/3014786‑3014923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑