| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,569 | A→C | I572L (ATC→CTC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,569 | 0 | A | C | 100.0% | 76.9 / NA | 20 | I572L (ATC→CTC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (9/11); total (9/11) | |||||||||||
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162497‑4162641 | cGAGAAGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCa > 1:539289/1‑76 (MQ=255) gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCaa > 2:116135/1‑76 (MQ=255) cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAActct < 1:299720/76‑1 (MQ=255) acCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTc > 2:46007/1‑76 (MQ=255) cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCgt > 2:258133/1‑76 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTa < 2:522911/76‑1 (MQ=255) cACTACGTTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACg > 1:449820/1‑76 (MQ=255) cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGca > 2:405213/1‑76 (MQ=255) gcgTATGTCCAATCGAAACCCCTGAAGATCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTaa < 1:632701/76‑1 (MQ=255) gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACg > 1:445359/1‑76 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGa < 1:319623/76‑1 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 2:174080/1‑76 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCGCAGACTAACGAAt < 1:635544/76‑1 (MQ=255) aaaCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt < 1:247777/76‑1 (MQ=255) aaaCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt < 1:466068/76‑1 (MQ=255) aCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg < 1:24414/76‑1 (MQ=255) ccTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga > 1:73526/1‑76 (MQ=255) gTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa < 1:172787/76‑1 (MQ=255) gAACATCGGTCTTCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGt < 2:18774/76‑1 (MQ=255) gTCTGCTCAACTCTCTGTCCGTGTGCGCACAGACTAGCGAGTACGGCTTCCTTGAGACTCCGTATCGTAAAGGGAc < 2:652788/76‑1 (MQ=255) cTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg < 2:206503/76‑1 (MQ=255) | CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162497‑4162641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |