Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,202,426	G→A	A45V (GCA→GTA)	iclR ←	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,202,426	0	G	A	100.0%	53.9 / NA	15	A45V (GCA→GTA)	iclR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/3);  total (12/3)

CTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAG  >  REL606/4202353‑4202483                                                                          |                                                          ctgTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTAcc                                                         <  1:316055/76‑1 (MQ=255)  tgTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTACca                                                        >  2:426815/1‑76 (MQ=255)       cATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCCCCAGTTCCGTGAGTACCACACTg                                                   >  1:96673/1‑76 (MQ=255)       cATTGTGGTTAGCAGGCGGTGGGTCGTCGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTACCACACTg                                                   >  1:685167/1‑76 (MQ=255)        aTTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTACCACACTGc                                                  >  2:217544/1‑76 (MQ=255)           gtgGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTACCACACTGCCAt                                               <  2:70706/76‑1 (MQ=255)                    aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTACCACACTGCCATTGGATTCgg                                      >  1:215743/1‑76 (MQ=255)                             gTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTACCACACTGCCATTGGATTCGGCAATCCACt                             >  1:712454/1‑76 (MQ=255)                                gTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTACCACACTGCCATTGGATTCGGCAATCCACTCCa                          >  1:594001/1‑76 (MQ=255)                                gTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTACCACACTGCCATTGGATTCGGCAATCCACTCCa                          <  2:146963/76‑1 (MQ=255)                                  ggAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTACCACACTGCCATTGGATTCGGCAATCCACTCCAGt                        >  2:404690/1‑76 (MQ=255)                                                cGGCTTGTTGCGCCAGTTCCGTGAGTACCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCAcg          >  1:609310/1‑76 (MQ=255)                                                     tgttgCGCCAGTTCCGTGAGTACCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTa     >  2:293750/1‑76 (MQ=255)                                                       ttgCGCCAGTTCCGTGAGTACCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTaaa   >  2:39736/1‑76 (MQ=255)                                                        tgCGCCAGTTCCGTGAGTACCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAg  >  1:362378/1‑76 (MQ=255)                                                                          |                                                          CTGTTGCATTGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAG  >  REL606/4202353‑4202483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑