Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	825,980	G→A	D96N (GAT→AAT)	dinG →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	825,980	0	G	A	100.0%	71.5 / NA	20	D96N (GAT→AAT)	dinG	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/11);  total (9/11)

CGCGAAGAGCAAAAAACGCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAGATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTAAATTCACTGCCGCTTTTGGGCGTGGGCGCTA  >  REL606/825911‑826047                                                                      |                                                                    cgcgAAGAGCAAAAAACGCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTAc                                                               <  1:632166/76‑1 (MQ=255)    gAAGAGCAAAAAACGCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCgc                                                            <  2:260/76‑1 (MQ=255)         gCAAAAAACGCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGatgctg                                                       >  2:712828/1‑76 (MQ=255)          cAAAAAACGCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGa                                                      >  1:445043/1‑76 (MQ=255)           aaaaaaCGCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGaa                                                     >  2:729/1‑76 (MQ=255)              aaaCGCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGaaaaa                                                  >  2:661411/1‑76 (MQ=255)                 cGCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGAt                                               <  1:142867/76‑1 (MQ=255)                  gCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATc                                              <  1:663859/76‑1 (MQ=255)                  gCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATc                                              <  1:554603/76‑1 (MQ=255)                  gCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATc                                              <  1:471609/76‑1 (MQ=255)                   atggtggTGAGTACNGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATCa                                             >  1:390750/2‑76 (MQ=255)                              tACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATCATTCCCGATCtt                                  <  1:712828/76‑1 (MQ=255)                                ccGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTaa                                <  1:358767/76‑1 (MQ=255)                                   ccAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTAAAtt                             >  2:13415/1‑76 (MQ=255)                                   ccAACGTGGCATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTAAAtt                             <  2:582151/76‑1 (MQ=255)                                            cATTGCAGGATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTAAATTCACTGCCGc                    >  1:334123/1‑76 (MQ=255)                                                    gATCAGATTTACAGCAAAAATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTAAATTCACTGCCGCTTTtgggc            <  2:517819/76‑1 (MQ=255)                                                             tACAGCAAAAATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTAAATTCACTGCCGCTTTTGGGCGTGGGCGCt   >  1:316150/1‑76 (MQ=255)                                                             tACAGCAAAAATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTAAATTCACTGCCGCTTTTGGGCGTGGGCGCt   >  1:202429/1‑76 (MQ=255)                                                              aCAGCAAAAATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTAAATTCACTGCCGCTTTTGGGCGTGGGCGCTa  <  1:694541/76‑1 (MQ=255)                                                                      |                                                                    CGCGAAGAGCAAAAAACGCTGGTGGTGAGTACCGCCAACGTGGCATTGCAGGATCAGATTTACAGCAAAGATTTACCGCTGCTGAAAAAGATCATTCCCGATCTTAAATTCACTGCCGCTTTTGGGCGTGGGCGCTA  >  REL606/825911‑826047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑