New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 448189815 (0.570)3 (0.120) 3/282 2.9 17.5% coding (1940/2856 nt) valS valyl‑tRNA synthetase
?NC_000913 = 4481970 14 (0.560)coding (1868/2856 nt) valS valyl‑tRNA synthetase
Rejected: Frequency below cutoff threshold.

GAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4481806‑4481898
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctggcgtCTACCGGTCGTGACATCAACTGGGATATGAAGCGTCTGGAAGGTTACCGTAACTTCTGTAACAAGCTGTGGAACGCCAGCCGCTTTGTGCTGATGAACACAGAAGGTCAGGATTGCGGCTTCAACGGCGGCGAAAT  <  NC_000913/4481970‑4481835
                                                                                                                                                                                                                                     
GAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGGCTACCGGTCCTGACATCAACTGGGATATGAAGCGTCTGGAAGGTTACCGTAACTT                                                                                   >  2:232655/1‑148
                   CGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTCACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGTCTACCGGTCGTGACCTCAACTGCGATATGATGCGTCAGGAAGGTTACCGTAACTTTCTTAACAACCTGTTGCACGC                                                              >  1:220242/1‑150
                                                                              CAAAGCGGCTGGCGTCTACCGGTCGTGACATCAACTGGGATATGAAGCGTCTGGAAGGTTCCCGTAACTTCTGTAACAAGCTGTGGAACGCCCGCCGCTTTGTGCTGATGAACACAGAAGGTCAGGATTGCGGCTTCAACGGCGGCGAAAT  >  2:43783/1‑151
                                                                                                                                                                                                                                     
GAATCCAGCGGTCCGCCAGCGACAGCGTCATTTCGCCGCCGTTGAAGCCGCAATCCTGACCTTCTGTGTTCATCAGCACAAAGCGGCTGGCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4481806‑4481898
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctggcgtCTACCGGTCGTGACATCAACTGGGATATGAAGCGTCTGGAAGGTTACCGTAACTTCTGTAACAAGCTGTGGAACGCCAGCCGCTTTGTGCTGATGAACACAGAAGGTCAGGATTGCGGCTTCAACGGCGGCGAAAT  <  NC_000913/4481970‑4481835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.