Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,318	0	T	G	55.0%	10.1 / 25.9	20	V538G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (4/5);  new base G (11/0);  total (15/5)
Fisher's exact test for biased strand distribution p-value = 8.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-03

CTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA  >  NC_000913/354186‑354457                                                                                                                                     |                                                                                                                                            cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGTGGGGGTTGTCATTCCGaa                                                                                                                           >  1:341617/1‑151 (MQ=255)   gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGGGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGaaaa                                                                                                                         >  2:57849/1‑150 (MQ=255)    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                                         <  2:316980/150‑1 (MQ=255)          tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGTGGGGAAAAATATGCTGGAAGGGCCGGGGGGGGGGGGGGTTGTCGTTCCGAAagagggggg                                                                                                                  >  2:228485/1‑146 (MQ=255)          tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGCAAGAGAGCgg                                                                                                                  >  1:161237/1‑150 (MQ=255)                         ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGGGCTGGAAGGGCAGGTGGGGGGGGGGTTTGTCATTCCGAAAGAGAGAGACAAGCTGGGAGATCg                                                                                                   >  2:362225/1‑151 (MQ=255)                          gCATCGGCTGGGGACGCGCGAGATTGAAGGGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGTGGGGGGAAAGAAGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAAAGAGGCAGAGGCTGGAAGAtggg                                                                                                  >  2:307691/1‑148 (MQ=255)                          gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                                                   <  2:215103/150‑1 (MQ=255)                                 cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                                           <  2:18472/151‑1 (MQ=255)                                              agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGGAGCGCTGGAAGGGCAGGGGGGGGGGGGGTTTGGCAGTTCGAAAGAGGGGGAAAGGGTGGGAGAAGGGGGGGGGGGGCACTCGCaaaa                                                                              >  2:383825/1‑149 (MQ=255)                                                            aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAAATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGAAAGTCTGGGAGAACGTGATGTGGCGCACTCGCAAGAGAAGGGGATTATgg                                                                >  1:39167/1‑151 (MQ=255)                                                            aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAAATGCGCCGGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGGAAGAGAGCGACAGGCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGGGATTATgg                                                                >  1:370864/1‑151 (MQ=255)                                                             tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGGGCCGTAAGGGCAGGTGGCGGGGGCGGTTGTTATTTCGAAAGAGAAGGGAAGTCTGGAAGAATGTGGGGGGGGGCACTTGCAAGAGAAGGGGATTATgg                                                                >  2:59962/1‑150 (MQ=255)                                                             tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGGTGCGCTGAAAGGGCAGGTGGGGGGGGCGGTTGGCCGTCCGAAAGAGAGCGACAGGCTGGGAGATAGGGCTGTGGCGGACCCGCCAGGGAAGGGGAATATggg                                                               >  1:138879/1‑150 (MQ=255)                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGAGGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGGGGCGCCCTCGCAAGAGAAGGCGGTTATGGGGCtggtggg                                                      >  2:50265/1‑150 (MQ=255)                                                                                              tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg                              >  2:136456/1‑151 (MQ=255)                                                                                                    gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg                         >  2:174764/1‑150 (MQ=255)                                                                                                          aaGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGt                  >  1:27127/1‑151 (MQ=255)                                                                                                              tGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTgg              <  1:228416/151‑1 (MQ=255)                                                                                                                           cAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTAGCGCACTCGCAAGGGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCaa  <  2:313923/150‑1 (MQ=255)                                                                                                                                     |                                                                                                                                            CTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA  >  NC_000913/354186‑354457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑