Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.76.9% 71.9 / 15.9 26pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (0/6);  new base . (9/11);  total (9/17)
Fisher's exact test for biased strand distribution p-value = 6.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCA  >  NC_000913/2173215‑2173506
                                                                                                                                                  |                                                                                                                                                 
gAAAAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGTTTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgc                                                                                                                                                >  2:374127/1‑148 (MQ=255)
       tttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTc                                                                                                                                      <  1:239190/151‑1 (MQ=255)
         tCCACGATGCACGGCGACCGCCAGCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGACACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTccc                                                                                                                                    <  2:387540/151‑1 (MQ=255)
               aTGCCCGGCGACCGCCATCGCCACGAGAAGGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACCCAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagcag                                                                                                                               <  1:88733/150‑1 (MQ=255)
                      gCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCg                                                                                                                       >  1:108413/1‑151 (MQ=255)
                               accaccACGAGGGAGCCGTTGGTGGCAAGATCGCCAAACGGCAGATCCTCATTACCTGGCACACAAACAGCAATTAAAAGGTTGAGTGGGATAAAAATCAGGCGTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                              <  1:208287/147‑1 (MQ=255)
                               aTCGCCCCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                              <  2:81370/151‑1 (MQ=255)
                                    cACGAAGACGCCGATGGTGGCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATc                                                                                                         <  1:410696/151‑1 (MQ=255)
                                                 aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAc                                                                                            <  1:371356/151‑1 (MQ=255)
                                                  tggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCCCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAcc                                                                                           <  1:36970/151‑1 (MQ=255)
                                                    gtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccg                                                                                          >  2:293873/1‑150 (MQ=255)
                                                                ccAAACGGCAGCACCGGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGGGGGAAAAAAATCAGGCTGGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc                                                                              <  2:276319/150‑1 (MQ=255)
                                                                             ccTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAACCGGGCTTGCGGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg                                                                <  1:78191/151‑1 (MQ=255)
                                                                             ccTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTaag                                                                >  2:109531/1‑149 (MQ=255)
                                                                             ccTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg                                                                >  2:387743/1‑151 (MQ=255)
                                                                                      ccGGCACACAAACAGCAATCAAAAGGGTGAGTGGGATAAAAACCAGGCTTCCCGATCCCCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt                                                       <  2:238346/151‑1 (MQ=255)
                                                                                         gCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAg                                                     >  1:218249/1‑150 (MQ=255)
                                                                                           ccccaAACAGCAATAAAAAGGGGGGGTGGGAAAAAAATCAGACTTCCCGAACCCCCCGCCGTATGTCCCAGCAGCAAAGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa                                                  <  2:133583/148‑1 (MQ=255)
                                                                                           acacaAACAGCAATTAAAAGGGTGAGTGGGAAAAAAATCAGGCTTGCGGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa                                                  <  1:178109/151‑1 (MQ=255)
                                                                                                  cAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGt                                           >  2:121979/1‑151 (MQ=255)
                                                                                                       ataaaaaTGGTGAGTGGGATAAAAATCAGGCTGGCGGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                      <  1:293793/148‑1 (MQ=255)
                                                                                                        ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                      >  1:201250/1‑150 (MQ=255)
                                                                                                                  aaggggaaaaaaacTCGGGCTTGCCGATCCCGCCGCCGTATGTCCGAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgat                            <  1:107229/141‑1 (MQ=255)
                                                                                                                     ggggAAAAAAACCAGGCTTGCGGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATggg                         <  1:339446/149‑1 (MQ=255)
                                                                                                                             aaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCa                 >  1:115589/1‑150 (MQ=255)
                                                                                                                             aaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCa                 >  1:278221/1‑150 (MQ=255)
                                                                                                                                          cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagc   <  1:374029/151‑1 (MQ=255)
                                                                                                                                              atccccccccCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAGTCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagca  <  2:369844/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCA  >  NC_000913/2173215‑2173506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: