| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,337,723 | 0 | A | C | 58.3% | 3.6 / 11.3 | 13 | G566G (GGT→GGG) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-02 | |||||||||||
CGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCG > NC_000913/2337628‑2337846 | cgACCGCGGTTGGAAAAGCAAAGAATAGGGTGGGGTGGTTTCCCCCCCAGCGTTGGGTCGAAAAAGTTTTCTTTTTTATTAGGTGGGGAATATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGt < 2:68399/151‑1 (MQ=255) ggAGAAGCACAGAATATGGTCGTGAGTGTCCGCCCCCAGCAGTCGGTCGAAAAAGTCTTCTTTTTAAATACTGGCGGAGAATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTg < 1:340591/150‑1 (MQ=255) gCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGACTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGagagag > 2:235764/1‑150 (MQ=255) gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa > 1:124581/1‑150 (MQ=255) gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa > 1:136244/1‑150 (MQ=255) tatGGTCGGGAGTGTTCGCCACCAGCAGTCGGTCGATAAGGTCTTCTTCTTAAATACGGGCGGAAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGAc < 1:156478/151‑1 (MQ=255) gttttCGCCCCCAGCAGTCGGTGAAAAAAGTCTTTTTTTTTAATACGGGCGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTcc < 2:359242/147‑1 (MQ=255) tgtTCGCCCCCAGCGGGCGGGCGATAGAGTCTTCTTTTTTAATGGGTGCGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCt < 1:48222/150‑1 (MQ=255) tgtCCGCCACCGGCGGTCGGTGAATAAAGTTTTTTTTTTTAATGCGTGCGGCAGTTTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCt < 1:38386/150‑1 (MQ=255) tccGCCCCCGGCGGTGGGTCGAAAAGGTTTTCTTCTTTAATGGGTGCGGGAGATTTTCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTggg < 2:172211/149‑1 (MQ=255) ctgtcggtcgATAAAGTCTTCTTCTTTAAGCCGGGCGGAAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGa < 1:258342/146‑1 (MQ=255) aaaGTTTTTTTTTTTAGTGAGTGCGGCATATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATg < 1:294281/151‑1 (MQ=255) tcttTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgcg > 2:333963/1‑147 (MQ=255) | CGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCG > NC_000913/2337628‑2337846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |